FN Archimer Export Format
PT J
TI Methanococcoides vulcani sp nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides
BT 
AF L HARIDON, Stephane
   CHALOPIN, Morgane
   COLOMBO, Delphine
   TOFFIN, Laurent
AS 1:1,2,3;2:1,2,3;3:1,2,3;4:1,2,3;
FF 1:;2:;3:;4:PDG-REM-EEP-LMEE;
C1 UBO, UEB, Inst Univ Europeen Mer IUEM UMR 6197, LMEE, F-29280 Plouzane, France.
   CNRS, IUEM UMR 6197, LMEE, F-29280 Plouzane, France.
   IFREMER, UMR 6197, LMEE, F-29280 Plouzane, France.
C2 UBO, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.511
TC 30
UR https://archimer.ifremer.fr/doc/00211/32181/30616.pdf
LA English
DT Article
CR MEDECO
BO Unknown
AB A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 degrees C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na+. The DNA G+C content of strain SLH33(T) was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33(T) within the genus Methanococcoides. The novel isolate was related most closely to Methanococcoides methylutens TMA-10(T) (98.8% 16S rRNA gene sequence similarity) but distantly related to Methanococcoides burtonii DSM 6242(T) (97.6%) and Methanococcoides alaskense AK-5(T) (97.6%). DNA DNA hybridization studies indicated that strain SLH33(T) represents a novel species, given that it shared less than 16% DNA DNA relatedness with Methanococcoides methylutens TMA-10(T). The name Methanococcoides vulcani sp. nov. is proposed for this novel species, with strain SLH33(T) (=DSM 26966(T)=JCM 19278(T)) as the type strain. An emended description of the genus Methanococcoides is also proposed.
PY 2014
PD JUL
SO International Journal Of Systematic And Evolutionary Microbiology
SN 1466-5026
PU Soc General Microbiology
VL 64
IS 6
UT 000340019200022
BP 1978
EP 1983
DI 10.1099/ijs.0.058289-0
ID 32181
ER

EF