FN Archimer Export Format
PT J
TI Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi
BT 
AF CASTILLO-LIZARDO, Melissa
   HENNEKE, Ghislaine
   VIGUERA, Enrique
AS 1:1;2:2,3;3:1;
FF 1:;2:PDG-REM-EEP-LMEE;3:;
C1 Univ Malaga, Fac Ciencias, Dept Biol Celular Genet & Fisiol, E-29071 Malaga, Spain.
   Univ Bretagne Occidentale, Inst Francais Rech Exploitat Mer, UMR 6197, Lab Microbiol Environm Extremes, Plouzane, France.
   CNRS, UMR 6197, Lab Microbiol Environm Extremes, Plouzane, France.
C2 UNIV MALAGA, SPAIN
   IFREMER, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-europe
IF 3.989
TC 18
UR https://archimer.ifremer.fr/doc/00212/32375/30835.pdf
   https://archimer.ifremer.fr/doc/00212/32375/30836.pdf
LA English
DT Article
DE ;slippage;primer-template misalignment;DNA polymerases;strand displacement activity;Archaea
AB Replication slippage or slipped-strand mispairing involves the misalignment of DNA strands during the replication of repeated DNA sequences, and can lead to genetic rearrangements such as microsatellite instability. Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA template carrying a hairpin structure and short direct repeats. We find that this occurs in both their wild-type (exo+) and exonuclease deficient (exo-) forms. The slippage behavior of PabPolB and PabPolD, probably due to limited strand displacement activity, resembles that observed for the high fidelity P furiosus (Pfu) DNA polymerase. The presence of PabPCNA inhibited PabPolB and PabPolD slippage. We propose a model whereby PabPCNA stimulates strand displacement activity and polymerase progression through the hairpin, thus permitting the error-free replication of repetitive sequences.
PY 2014
PD AUG
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Research Foundation
VL 5
IS 403
UT 000341515800001
BP 1
EP 10
DI 10.3389/fmicb.2014.00403
ID 32375
ER

EF