Effect of the probiotic strain Phaeobacter gallaeciensis after bacterial challenge on the complete larval development of Pecten maximus

Type Article
Date 2014-01
Language English
Author(s) Genard Bertrand1, Larouche Olivier1, Nicolas Jean-Louis2, Miner Philippe2, Beaudin Marie-Lou1, Tremblay Rejean1
Affiliation(s) 1 : Univ Quebec, Inst Sci Mer, Rimouski, PQ G5L 3A1, Canada.
2 : Ctr Bretagne, CNRS UBO IRD Ifremer, UR PFOM, UMR LEMAR 6539, FR-29280 Plouzane, France.
Source Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2014-01 , Vol. 27 , N. 1 , P. 27-34
DOI 10.1051/alr/2014005
WOS© Times Cited 8
Keyword(s) Probiotics, hatchery, bacterial challenge, larval development, Pecten maximus, Phaeobacter gallaeciensis, Vibrio
Abstract The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival rates, shell lengths and metamorphosis ability. Moreover, antioxidant (catalase and superoxide dismutase) and lipids peroxidation activities were also measured after bacterial challenge in order to evaluate the physiological response of larvae to pathogen exposition. Our results indicated an activation of the two selected antioxidant enzymes after bacterial challenge, but the increase was significantly lower in probiotic treated larvae. At the end of the experiment, the strain X34 treatment prevented a mass mortality event and showed a significant increase in the number of individuals reaching competence, when compared to untreated larvae.
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Genard Bertrand, Larouche Olivier, Nicolas Jean-Louis, Miner Philippe, Beaudin Marie-Lou, Tremblay Rejean (2014). Effect of the probiotic strain Phaeobacter gallaeciensis after bacterial challenge on the complete larval development of Pecten maximus. Aquatic Living Resources, 27(1), 27-34. Publisher's official version : https://doi.org/10.1051/alr/2014005 , Open Access version : https://archimer.ifremer.fr/doc/00213/32429/