FN Archimer Export Format
PT J
TI Effect of the probiotic strain Phaeobacter gallaeciensis after bacterial challenge on the complete larval development of Pecten maximus
BT 
AF GENARD, Bertrand
   LAROUCHE, Olivier
   NICOLAS, Jean-Louis
   MINER, Philippe
   BEAUDIN, Marie-Lou
   TREMBLAY, Rejean
AS 1:1;2:1;3:2;4:2;5:1;6:1;
FF 1:;2:;3:PDG-RBE-PFOM-PI;4:PDG-RBE-PFOM-PI;5:;6:;
C1 Univ Quebec, Inst Sci Mer, Rimouski, PQ G5L 3A1, Canada.
   Ctr Bretagne, CNRS UBO IRD Ifremer, UR PFOM, UMR LEMAR 6539, FR-29280 Plouzane, France.
C2 UNIV QUEBEC, CANADA
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 1.014
TC 8
UR https://archimer.ifremer.fr/doc/00213/32429/30895.pdf
LA English
DT Article
DE ;Probiotics;hatchery;bacterial challenge;larval development;Pecten maximus;Phaeobacter gallaeciensis;Vibrio
AB The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival rates, shell lengths and metamorphosis ability. Moreover, antioxidant (catalase and superoxide dismutase) and lipids peroxidation activities were also measured after bacterial challenge in order to evaluate the physiological response of larvae to pathogen exposition. Our results indicated an activation of the two selected antioxidant enzymes after bacterial challenge, but the increase was significantly lower in probiotic treated larvae. At the end of the experiment, the strain X34 treatment prevented a mass mortality event and showed a significant increase in the number of individuals reaching competence, when compared to untreated larvae.
PY 2014
PD JAN
SO Aquatic Living Resources
SN 0990-7440
PU Edp Sciences S A
VL 27
IS 1
UT 000341699500003
BP 27
EP 34
DI 10.1051/alr/2014005
ID 32429
ER

EF