Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids

Type Article
Date 2016-05
Language English
Author(s) Batista Frederico M.1, 2, Lopez-Sanmartin Monserrat3, Boudry PierreORCID4, Navas Jose I.4, Ruano Francisco5, Renault Tristan6, Fonseca Vera G.2, 7, Leitao Alexandra1, 8
Affiliation(s) 1 : Inst Portugues Mar & Atmosfera, Estacao Expt Moluscicultura Tavira, Div Aquicultura & Valorizacao, Av 5 Outubro, P-8700305 Olhao, Portugal.
2 : Univ Algarve, Ctr Ciencias Mar CCMAR, Campus Gambelas, P-8005139 Faro, Portugal.
3 : Inst Invest & Formac Agr Pesquera IFAPA, Ctr Agua Pino, Cartaya 21450, Spain.
4 : IFREMER, UMR LEMAR, F-29280 Plouzane, France.
5 : Inst Portugues Mar & Atmosfera, Div Aquicultura & Valorizacao, Av Brasilia, P-1449006 Lisbon, Portugal.
6 : IFREMER, LGPMM SG2M, Ave Mus Loup, F-17930 La Tremblade, France.
7 : Zool Res Museum Alexander Koenig ZFMK, Ctr Mol Biodivers Res, Adenauerallee 160, D-53113 Bonn, Germany.
8 : Qatar Univ, Ctr Environm Studies, POB 2713, Doha, Qatar.
Source Aquaculture Research (1355-557X) (Wiley-blackwell), 2016-05 , Vol. 47 , N. 5 , P. 1530-1536
DOI 10.1111/are.12613
WOS© Times Cited 1
Keyword(s) aneuploidy, ostreid herpesvirus 1, oyster, Crassostrea
Abstract Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the OsHV-1 reference type. Moreover, in situ hybridization confirmed the presence of OsHV-1 in gill tissue. Oysters where OsHV-1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to OsHV-1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.
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Batista Frederico M., Lopez-Sanmartin Monserrat, Boudry Pierre, Navas Jose I., Ruano Francisco, Renault Tristan, Fonseca Vera G., Leitao Alexandra (2016). Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids. Aquaculture Research, 47(5), 1530-1536. Publisher's official version : https://doi.org/10.1111/are.12613 , Open Access version : https://archimer.ifremer.fr/doc/00215/32581/