FN Archimer Export Format
PT J
TI Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
BT 
AF BATISTA, Frederico M.
   LOPEZ-SANMARTIN, Monserrat
   BOUDRY, Pierre
   NAVAS, Jose I.
   RUANO, Francisco
   RENAULT, Tristan
   FONSECA, Vera G.
   LEITAO, Alexandra
AS 1:1,2;2:3;3:4;4:4;5:5;6:6;7:2,7;8:1,8;
FF 1:;2:;3:PDG-RBE-PFOM;4:;5:;6:PDG-RBE-SG2M-LGPMM;7:;8:;
C1 Inst Portugues Mar & Atmosfera, Estacao Expt Moluscicultura Tavira, Div Aquicultura & Valorizacao, Av 5 Outubro, P-8700305 Olhao, Portugal.
   Univ Algarve, Ctr Ciencias Mar CCMAR, Campus Gambelas, P-8005139 Faro, Portugal.
   Inst Invest & Formac Agr Pesquera IFAPA, Ctr Agua Pino, Cartaya 21450, Spain.
   IFREMER, UMR LEMAR, F-29280 Plouzane, France.
   Inst Portugues Mar & Atmosfera, Div Aquicultura & Valorizacao, Av Brasilia, P-1449006 Lisbon, Portugal.
   IFREMER, LGPMM SG2M, Ave Mus Loup, F-17930 La Tremblade, France.
   Zool Res Museum Alexander Koenig ZFMK, Ctr Mol Biodivers Res, Adenauerallee 160, D-53113 Bonn, Germany.
   Qatar Univ, Ctr Environm Studies, POB 2713, Doha, Qatar.
C2 IPMA, PORTUGAL
   UNIV ALGARVE, PORTUGAL
   IFAPA, SPAIN
   IFREMER, FRANCE
   IPMA, PORTUGAL
   IFREMER, FRANCE
   ZFMK, GERMANY
   UNIV QATAR, QATAR
SI BREST
   NANTES
SE PDG-RBE-PFOM
   PDG-RBE-SG2M-LGPMM
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-europe
   copubli-int-hors-europe
IF 1.461
TC 7
UR https://archimer.ifremer.fr/doc/00215/32581/35759.pdf
LA English
DT Article
DE ;aneuploidy;ostreid herpesvirus 1;oyster;Crassostrea
AB Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the OsHV-1 reference type. Moreover, in situ hybridization confirmed the presence of OsHV-1 in gill tissue. Oysters where OsHV-1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to OsHV-1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.
PY 2016
PD MAY
SO Aquaculture Research
SN 1355-557X
PU Wiley-blackwell
VL 47
IS 5
UT 000373793200015
BP 1530
EP 1536
DI 10.1111/are.12613
ID 32581
ER

EF