FN Archimer Export Format
PT J
TI Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure
BT 
AF LE GOIC, Nelly
   HEGARET, Helene
   BOULAIS, Myrina
   BEGUEL, Jean-Philippe
   LAMBERT, Christophe
   FABIOUX, Caroline
   SOUDANT, Philippe
AS 1:1;2:1;3:2;4:1;5:1;6:1;7:1;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Laboratoire des Sciences de l'Environnement MARin (LEMAR), IUEM, Plouzané, France
   Laboratoire de Physiologie des Invertébrés, Station Expérimentale d'Argenton, Argenton, France
C2 UBO, FRANCE
   UBO, FRANCE
SI BREST
SE UMR-LEMAR
   IUEM
IF 2.928
TC 29
UR https://archimer.ifremer.fr/doc/00216/32760/31321.pdf
LA English
DT Article
DE ;Pacific oyster;gamete quality;ROS production;viability;flow cytometry;ecotoxicology
AB The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. 
PY 2014
PD DEC
SO Cytometry Part A
SN 1552-4922
PU Wiley-blackwell
VL 85A
IS 12
UT 000345344700010
BP 1049
EP 1056
DI 10.1002/cyto.a.22577
ID 32760
ER

EF