FN Archimer Export Format
PT J
TI Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas.
BT 
AF POIRIER, Aurore C.
   SCHMITT, Paulina
   ROSA, Rafael D.
   VANHOVE, Audrey S.
   KIEFFER-JAQUINOD, Sylvie
   RUBIO, Tristan P.
   CHARRIERE, Guillaume M.
   DESTOUMIEUX-GARZON, Delphine
AS 1:1,2;2:1,2,3,5;3:1,2;4:1,2;5:4;6:1,2;7:1,2;8:1,2;
FF 1:;2:PDG-RBE-BOME-LALR;3:;4:;5:;6:;7:;8:;
C1 Univ Montpellier I, Univ Montpellier 2, IFREMER, Lab Ecol Coastal Marine Syst,CNRS UMR 5119, F-34095 Montpellier, France.
   IRD, F-34095 Montpellier, France.
   Pontificia Univ Catolica Valparaiso, Inst Biol, Lab Genet & Inmunol Mol, Valparaiso 2373223, Chile.
   Univ Grenoble 1, CEA, INSERM, U1038,Etud Dynam Proteomes,Lab Biol Grande Echell, F-38054 Grenoble 9, France.
   IFREMER
C2 UNIV MONTPELLIER, FRANCE
   IRD, FRANCE
   UNIV VALPARAISO, CHILE
   UNIV GRENOBLE, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
   PALAVAS
SE PDG-RBE-BOME
   PDG-RBE-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 4.573
TC 60
UR https://archimer.ifremer.fr/doc/00217/32866/31784.pdf
LA English
DT Article
DE ;Antimicrobial Peptide (AMP);DNA;Innate Immunity;Invertebrate;Reactive Oxygen Species (ROS);NET;Mollusk
AB Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial tissues surrounding wounds, whereas they were absent from tissues of unchallenged oysters. Consistently, antimicrobial histones were found to accumulate in oyster tissues following injury or infection with vibrios. Finally, oyster ET formation was highly dependent on the production of reactive oxygen species by hemocytes. This shows that ET formation relies on common cellular and molecular mechanisms from vertebrates to invertebrates. Altogether, our data reveal that ET formation is a defense mechanism triggered by infection and tissue damage, which is shared by relatively distant species suggesting either evolutionary conservation or convergent evolution within Bilateria.
PY 2014
PD SEP
SO Journal Of Biological Chemistry
SN 0021-9258
PU Amer Soc Biochemistry Molecular Biology Inc
VL 289
IS 36
UT 000341985300009
BP 24821
EP 24831
DI 10.1074/jbc.M114.576546
ID 32866
ER

EF