FN Archimer Export Format
PT C
TI Autophagy in Pacific oyster, Crassostrea gigas
BT 
AF MOREAU, Pierrick
   MOREAU, Kevin
   MORGA, Benjamin
   TOURBIEZ, Delphine
   TRAVERS, Marie-Agnes
   RUBINSZTEIN, David
   RENAULT, Tristan
AS 1:1;2:2;3:1;4:1;5:1;6:2;7:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-SG2M-LGPMM;5:PDG-RBE-SG2M-LGPMM;6:;7:PDG-RBE-SG2M;
C1 IFREMER, SG2M-LGPMM, Avenue Mus de Loup, F-17390 La Tremblade, France
   Department of Medical Genetics, Cambridge Institute for Medical Research, Cambridge CB2 OXY, England, UK
C2 IFREMER, FRANCE
   UNIV CAMBRIDGE, UK
SI LA TREMBLADE
   CAMBRIDGE, UK.
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE-SG2M
UR https://archimer.ifremer.fr/doc/00218/32876/31345.pdf
LA English
DT Poster
DE ;Autophagy;Pacific oyster;Crassostrea gigas;OsHV-1;Vibrio aestuarianus
AB Mass mortality outbreaks of Pacific oysters, Crassostrea gigas, are reported in different areas around the world affecting seriously the shellfish aquaculture sector. Two pathogens are associated with these mortality outbreaks, ostreid herpesvirus 1 (OsHV-1) and Vibrio aestuarianus. In this contexte, a better knowledge is needed in terms of oyster immunity. For this purpose, as an important degradation pathway autophagy was investigated in the Pacific oysters. An in silico research of genes involved in autophagy using the C. gigas genome allowed identification of homologs of ATG1 and ATG8/LC3(Microtubule-associated protein 1A/1B-light chain 3). Gene expression and protein detection were analysed using real-time PCR and western blot, respectively. ATG1 and ATG8 gene expression was upregulated during an experimental viral infection. Western blot analysis showed an increase of LC3 protein during the infection, suggesting an activation of autophagy. Ammonium chloride treatment was associated with increased oyster mortality whereas less mortality was reported after carbamazepine treatment in experimentally infected animals. Results suggest a protective role of autophagy against OsHV-1 and V. aestuarianus infection. This study contributes to better understand the innate immune system of Pacific oysters.
PY 2014
PD SEP
ID 32876
ER

EF