Characterization and Function of the First Antibiotic Isolated from a Vent Organism: The Extremophile Metazoan Alvinella pompejana

Type Article
Date 2014-04
Language English
Author(s) Tasiemski Aurelie1, Jung Sascha2, Boidin-Wichlacz Celine1, Jollivet Didier3, Cuvillier-Hot Virginie1, Pradillon Florence4, Vetriani Costantino5, 6, Hecht Oliver2, Soennichsen Frank D.7, Gelhaus Christoph8, Hung Chien-Wen9, Tholey Andreas9, Leippe Matthias8, Groetzinger Joachim2, Gaill Francoise10
Affiliation(s) 1 : Univ Lille 1, CNRS, UMR8198, Lab GEPV, F-59655 Villeneuve Dascq, France.
2 : Univ Kiel, Inst Biochem, Kiel, Germany.
3 : Univ Paris 06, CNRS, UMR7144, Stn Biol,Lab Adaptat & Biol Invertebre Condit Ext, Roscoff, France.
4 : IFREMER, Ctr Brest, REM EEP LEP, Plouzane, France.
5 : Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08903 USA.
6 : Rutgers State Univ, Inst Marine & Coastal Sci, New Brunswick, NJ 08903 USA.
7 : Univ Kiel, Otto Diels Inst Organ Chem, Kiel, Germany.
8 : Univ Kiel, Inst Zool, D-24098 Kiel, Germany.
9 : Univ Kiel, Div Systemat Proteome Res, Inst Expt Med, Kiel, Germany.
10 : Univ Paris 06, CNRS, BOREA IRD, Museum Natl Hist Nat, Paris, France.
Source Plos One (1932-6203) (Public Library Science), 2014-04 , Vol. 9 , N. 4 , P. 1-10
DOI 10.1371/journal.pone.0095737
WOS© Times Cited 14
Abstract The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion, alvinellacin shapes and controls the worm's epibiotic microflora. Our results provide insights into the nature of an abyssal antimicrobial peptide (AMP) and into the manner in which an extremophile eukaryote uses it to interact with the particular microbial community of the hydrothermal vent ecosystem. Unlike earlier studies done on hydrothermal vents that all focused on the microbial side of the symbiosis, our work gives a view of this interaction from the host side.
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Tasiemski Aurelie, Jung Sascha, Boidin-Wichlacz Celine, Jollivet Didier, Cuvillier-Hot Virginie, Pradillon Florence, Vetriani Costantino, Hecht Oliver, Soennichsen Frank D., Gelhaus Christoph, Hung Chien-Wen, Tholey Andreas, Leippe Matthias, Groetzinger Joachim, Gaill Francoise (2014). Characterization and Function of the First Antibiotic Isolated from a Vent Organism: The Extremophile Metazoan Alvinella pompejana. Plos One, 9(4), 1-10. Publisher's official version : https://doi.org/10.1371/journal.pone.0095737 , Open Access version : https://archimer.ifremer.fr/doc/00219/33052/