Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection

Type Article
Date 2015-01
Language English
Author(s) Le Bris Cedric1, Richard Gaelle1, Paillard Christine1, Lambert Christophe1, Seguineau Catherine2, Gauthier Olivier1, Pernet FabriceORCID2, Guerard Fabienne1
Affiliation(s) 1 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR IUEM UBO, UMR CNRS UBO IRD IFREMER 6539, F-29280 Plouzane, France.
2 : IFREMER, Lab Physiol Invertebres, LEMAR IUEM UBO, UMR CNRS UBO IRD IFREMER 6539, F-29280 Plouzane, France.
Source Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2015-01 , Vol. 42 , N. 1 , P. 16-24
DOI 10.1016/j.fsi.2014.10.021
WOS© Times Cited 19
Keyword(s) Venerupis philippinarum, Vibrio tapetis, Immunity, Phenoloxidase, Superoxide dismutase
Abstract Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection. This first response in the mantle could be explained by the proximity to the injection region (extrapallial cavity). In the hemolymph the response occurred at later times and was associated with an increase in PO activity and a decrease in SOD activity. As hemolymph is a circulating fluid, this response delay could be due to an “integration time” needed by the organism to counteract the infection. Injections also impacted PO and SOD activities in both tissues and confirmed a difference in pathogenicity between the two V. tapetis strains.
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Le Bris Cedric, Richard Gaelle, Paillard Christine, Lambert Christophe, Seguineau Catherine, Gauthier Olivier, Pernet Fabrice, Guerard Fabienne (2015). Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection. Fish & Shellfish Immunology, 42(1), 16-24. Publisher's official version : https://doi.org/10.1016/j.fsi.2014.10.021 , Open Access version : https://archimer.ifremer.fr/doc/00221/33257/