FN Archimer Export Format PT J TI High or low dietary carbohydrate: protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout BT AF GEURDEN, I. MENNIGEN, J. PLAGNES-JUAN, E. VERON, V. CEREZO, T. MAZURAIS, David ZAMBONINO-INFANTE, Jose-Luis GATESOUPE, Joel SKIBA-CASSY, S. PANSERAT, S. AS 1:1;2:1;3:1;4:1;5:1;6:2;7:2;8:1,2;9:1;10:1; FF 1:;2:;3:;4:;5:;6:PDG-RBE-PFOM-ARN;7:PDG-RBE-PFOM-ARN;8:;9:;10:; C1 INRA, Nutr Metab & Aquaculture UR1067, F-64310 St Pee Sur Nivelle, France. IFREMER, UMR LEMAR 6539, Lab Adaptat Reprod & Nutr Poissons ARN, Lab Sci Environm Marin, F-29280 Plouzane, France. C2 INRA, FRANCE IFREMER, FRANCE SI BREST SE PDG-RBE-PFOM-ARN IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 IF 2.897 TC 81 UR https://archimer.ifremer.fr/doc/00221/33264/35594.pdf LA English DT Article DE ;Nutritional programming;Rainbow trout;Carbohydrates;Protein;Metabolism AB Based on the concept of nutritional programming in mammals, we tested whether an acute hyperglucidic-hypoproteic stimulus during first feeding could induce long-term changes in nutrient metabolism in rainbow trout. Trout alevins received during the five first days of exogenous feeding either a hyperglucidic (40% gelatinized starch + 20% glucose) and hypoproteic (20%) diet (VLP diet) or a high-protein (60%) glucose-free diet (HP diet, control). Following a common 105-day period on a commercial diet, both groups were then challenged (65 days) with a carbohydrate-rich diet (28%). Short-and long-term effects of the early stimuli were evaluated in terms of metabolic marker gene expressions and intestinal microbiota as initial gut colonisation is essential for regulating the development of the digestive system. In whole alevins (short term), diet VLP relative to HP rapidly increased gene expressions of glycolytic enzymes, while those involved in gluconeogenesis and amino acid catabolism decreased. However, none of these genes showed persistent molecular adaptation in the liver of challenged juveniles (long term). By contrast, muscle of challenged juveniles subjected previously to the VLP stimulus displayed downregulated expression of markers of glycolysis and glucose transport (not seen in the short term). These fish also had higher plasma glucose (9 h postprandial), suggesting impaired glucose homeostasis induced by the early stimulus. The early stimulus did not modify the expression of the analysed metabolism-related microRNAs, but had short-and long-term effects on intestinal fungi (not bacteria) profiles. In summary, our data show that a short hyperglucidic-hypoproteic stimulus during early life may have a long-term influence on muscle glucose metabolism and intestinal microbiota in trout. PY 2014 PD OCT SO Journal Of Experimental Biology SN 0022-0949 PU Company Of Biologists Ltd VL 217 IS 19 UT 000342506700009 BP 3396 EP 3406 DI 10.1242/jeb.106062 ID 33264 ER EF