FN Archimer Export Format
PT J
TI A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella
BT 
AF ROLLAND, Jean-Luc
   MEDHIOUB, Walid
   VERGNES, Agnes
   ABI-KHALIL, Celina
   SAVAR, Veronique
   ABADIE, Eric
   MASSERET, Estelle
   AMZIL, Zouher
   LAABIR, Mohamed
AS 1:1;2:2;3:1;4:1;5:3;6:4;7:5;8:3;9:5;
FF 1:PDG-RBE-BOME-LALR;2:;3:PDG-RBE-BOME-LALR;4:;5:PDG-ODE-LITTORAL-PHYC;6:PDG-ODE-LITTORAL-LERLR;7:;8:PDG-ODE-LITTORAL-PHYC;9:;
C1 IFREMER, Univ Montpellier 2,UMR Ecol Syst Marins Cotiers 5119, CNRS,IRD,UM1, F-34095 Montpellier 5, France.
   INSTM, Lab Aquaculture, Salammbo 2025, Tunisia.
   IFREMER, Lab Phycotoxines PHYC, F-44311 Nantes 3, France.
   IFREMER, Dept Labs Cotiers Environm Littoral & Ressouces A, F-34203 Sete, France.
C2 IFREMER, FRANCE
   INSTM, TUNISIA
   IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
SI SMEL SETE
   MONTPELLIER
   NANTES
   SETE
SE PDG-RBE-BOME-LALR
   PDG-ODE-LITTORAL-PHYC
   PDG-ODE-LITTORAL-LERLR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.853
TC 6
UR https://archimer.ifremer.fr/doc/00225/33606/32003.pdf
LA English
DT Article
DE ;shellfish;toxin;biomarker;expression;phytoplankton
AB To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in caspase-3/7 activity and normal percentage of apoptotic cells in this tissue. Taken together, these results suggest a feedback mechanism, which may occur in the digestive gland where BI.1 could play a key role in preventing the induction of apoptosis by PSTs. Moreover, the expression of CYP1A, Bax and BI.1 were found to be significantly correlated to the occurrence of natural toxic events, suggesting that the expression of these genes together could be used as biomarker to assess the biological responses of oysters to stress caused by PSTs.
PY 2014
PD SEP
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 12
IS 9
UT 000342902500019
BP 5035
EP 5054
DI 10.3390/md12095035
ID 33606
ER

EF