Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization

Type Article
Date 2015-02
Language English
Author(s) Barranger Audrey1, 2, Benabdelmouna Abdellah1, Degremont LionelORCID1, Burgeot Thierry2, Akcha Farida2
Affiliation(s) 1 : Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France
2 : Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l’Ile d’Yeu, BP 21105, 44311 Nantes Cedex 03, France
Source Aquatic Toxicology (0166-445X) (Elsevier), 2015-02 , Vol. 159 , P. 36-43
DOI 10.1016/j.aquatox.2014.11.011
WOS© Times Cited 10
Keyword(s) Aneuploidy, FISH, Diuron, Crassostrea gigas
Abstract Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in situ hybridization (FISH) was used to further characterize diuron-induced DNA damage at the chromosomal level. rDNA genes (5S and 18-5.8-28S), previously mapped onto C. gigas chromosomes 4, 5 and 10, were used as probes on the interphase nuclei of embryo preparations. Our results conclusively show higher aneuploidy (hypo- or hyperdiploidy) level in embryos from diuron-exposed genitors, with damage to the three studied chromosomal regions. This study suggests that sexually-developing oysters are vulnerable to diuron exposure, incurring a negative impact on reproductive success and oyster recruitment.
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Barranger Audrey, Benabdelmouna Abdellah, Degremont Lionel, Burgeot Thierry, Akcha Farida (2015). Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization. Aquatic Toxicology, 159, 36-43. Publisher's official version : https://doi.org/10.1016/j.aquatox.2014.11.011 , Open Access version : https://archimer.ifremer.fr/doc/00238/34911/