FN Archimer Export Format
PT J
TI Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization
BT 
AF BARRANGER, Audrey
   BENABDELMOUNA, Abdellah
   DEGREMONT, Lionel
   BURGEOT, Thierry
   AKCHA, Farida
AS 1:1,2;2:1;3:1;4:2;5:2;
FF 1:PDG-RBE-BE-LBEX;2:PDG-RBE-SG2M-LGPMM;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-BE;5:PDG-RBE-BE-LBEX;
C1 Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France
   Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l’Ile d’Yeu, BP 21105, 44311 Nantes Cedex 03, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   LA TREMBLADE
SE PDG-RBE-BE-LBEX
   PDG-RBE-SG2M-LGPMM
   PDG-RBE-BE
IN WOS Ifremer jusqu'en 2018
IF 3.557
TC 17
UR https://archimer.ifremer.fr/doc/00238/34911/33216.pdf
LA English
DT Article
DE ;Aneuploidy;FISH;Diuron;Crassostrea gigas
AB Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in situ hybridization (FISH) was used to further characterize diuron-induced DNA damage at the chromosomal level. rDNA genes (5S and 18-5.8-28S), previously mapped onto C. gigas chromosomes 4, 5 and 10, were used as probes on the interphase nuclei of embryo preparations. Our results conclusively show higher aneuploidy (hypo- or hyperdiploidy) level in embryos from diuron-exposed genitors, with damage to the three studied chromosomal regions. This study suggests that sexually-developing oysters are vulnerable to diuron exposure, incurring a negative impact on reproductive success and oyster recruitment.
PY 2015
PD FEB
SO Aquatic Toxicology
SN 0166-445X
PU Elsevier
VL 159
UT 000349592100004
BP 36
EP 43
DI 10.1016/j.aquatox.2014.11.011
ID 34911
ER

EF