FN Archimer Export Format
PT J
TI Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives
BT 
AF COLLETER, Julie
   PENMAN, David J.
   LALLEMENT, Stephane
   FAUVEL, Christian
   HANEBREKKE, Tanja
   OSVIK, Renate D.
   EILERTSEN, Hans C.
   D'COTTA, Helena
   CHATAIN, Beatrice
   PERUZZI, Stefano
AS 1:1,2;2:3;3:2;4:2;5:4;6:4;7:4;8:1;9:2;10:4;
FF 1:;2:;3:PDG-RBE-BOME-LALR;4:PDG-RBE-BOME-LALR;5:;6:;7:;8:;9:PDG-RBE-BOME-LALR;10:;
C1 Cirad, Persyst, UMR Intrepid, Montpellier, France.
   IFREMER, Lab Rech Piscicole Mediterranee, Stn Expt Aquaculture, Palavas Les Flots, France.
   Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland.
   Univ Tromso, Fac Biosci Fisheries & Econ, Dept Arctic & Marine Biol, Tromso, Norway.
C2 CIRAD, FRANCE
   IFREMER, FRANCE
   UNIV STIRLING, UK
   UNIV TROMSO, NORWAY
SI PALAVAS
SE PDG-RBE-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
IF 3.234
TC 12
UR https://archimer.ifremer.fr/doc/00239/34996/33531.pdf
   https://archimer.ifremer.fr/doc/00239/34996/33532.pdf
   https://archimer.ifremer.fr/doc/00239/34996/33533.pdf
   https://archimer.ifremer.fr/doc/00239/34996/33534.pdf
   https://archimer.ifremer.fr/doc/00239/34996/33535.pdf
LA English
DT Article
AB Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (gamma), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm(-2)), only one dose (60 mJ.cm(-2).min(-1) with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.
PY 2014
PD OCT
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 10
UT 000343942100030
BP 1
EP 11
DI 10.1371/journal.pone.0109572
ID 34996
ER

EF