Development and characterization of microsatellite markers for blackfin tuna (Thunnus atlanticus) with the use of Illumina paired-end sequencing

Type Article
Date 2014-10
Language English
Other localization http://fishbull.noaa.gov/1124/1124toc.htm
Author(s) Antoni Luca1, Luque Patricia L.1, Naghshpour Kaylie1, Reynal LionelORCID2, Saillant Eric1
Affiliation(s) 1 : Univ So Mississippi, Gulf Coast Res Lab, Ocean Springs, MS 39564 USA.
2 : IFREMER, Inst Francais Rech Exploitat Mer, Unite Biodiversite & Environm Martinique, F-97231 Le Robert, Martinique, France.
Source Fishery Bulletin (0090-0656) (Natl Marine Fisheries Service Scientific Publ Office), 2014-10 , Vol. 112 , N. 4 , P. 322-325
DOI 10.7755/FB.112.4.8
WOS© Times Cited 2
Abstract Twenty homologous microsatellite markers, or simple sequence repeats (SSRs), were developed for blackfin tuna (Thunnus atlanticus) through the use of a direct Seq-to-SSR approach. The number of alleles per locus ranged between 5 and 26, and the expected heterozygosity ranged between 0.640 and 0.969. Three loci displayed significant departure from Hardy-Weinberg equilibrium expectations, likely reflecting occurrence of null alleles. Another locus showed consecutive alleles that differed by one base pair only. Consequently, this locus may be prone to elevated rates of scoring errors. The remaining 16 loci will be valuable for studies in conservation genetics of blackfin tuna.
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Antoni Luca, Luque Patricia L., Naghshpour Kaylie, Reynal Lionel, Saillant Eric (2014). Development and characterization of microsatellite markers for blackfin tuna (Thunnus atlanticus) with the use of Illumina paired-end sequencing. Fishery Bulletin, 112(4), 322-325. Publisher's official version : https://doi.org/10.7755/FB.112.4.8 , Open Access version : https://archimer.ifremer.fr/doc/00239/34999/