High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas

Type Article
Date 2015-04
Language English
Author(s) Rosa Rafael D.1, Alonso Pascal2, Santini Adrien1, Vergnes Agnes2, Bachere Evelyne2
Affiliation(s) 1 : Ifremer, CNRS, Université Montpellier 2, IRD, Université Montpellier 1, UMR5119 - Ecology of Coastal Marine Systems, Place Eugène Bataillon, CC80, 34095 Montpellier, France
2 : Ifremer, France
Source Developmental & Comparative Immunology (0145-305X) (Elsevier), 2015-04 , Vol. 49 , N. 2 , P. 231-238
DOI 10.1016/j.dci.2014.12.002
WOS© Times Cited 19
Keyword(s) Mollusk bivalve, Antimicrobial peptide, Polymorphism, Gene copy number variation, High-throughput microfluidic RT-qPCR, Vibrio infection
Abstract We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses.
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Rosa Rafael D., Alonso Pascal, Santini Adrien, Vergnes Agnes, Bachere Evelyne (2015). High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas. Developmental & Comparative Immunology, 49(2), 231-238. Publisher's official version : https://doi.org/10.1016/j.dci.2014.12.002 , Open Access version : https://archimer.ifremer.fr/doc/00239/35009/