FN Archimer Export Format
PT J
TI High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas
BT 
AF ROSA, Rafael D.
   ALONSO, Pascal
   SANTINI, Adrien
   VERGNES, Agnes
   BACHERE, Evelyne
AS 1:1;2:2;3:1;4:2;5:2;
FF 1:;2:PDG-RBE-BOME-LALR;3:;4:PDG-RBE-IHPE;5:PDG-RBE-IHPE;
C1 Ifremer, CNRS, Université Montpellier 2, IRD, Université Montpellier 1, UMR5119 - Ecology of Coastal Marine Systems, Place Eugène Bataillon, CC80, 34095 Montpellier, France
   Ifremer, France
C2 UNIV MONTPELLIER, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-RBE-BOME-LALR
   PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.62
TC 25
UR https://archimer.ifremer.fr/doc/00239/35009/33545.pdf
LA English
DT Article
DE ;Mollusk bivalve;Antimicrobial peptide;Polymorphism;Gene copy number variation;High-throughput microfluidic RT-qPCR;Vibrio infection
AB We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses.
PY 2015
PD APR
SO Developmental & Comparative Immunology
SN 0145-305X
PU Elsevier
VL 49
IS 2
UT 000349880500004
BP 231
EP 238
DI 10.1016/j.dci.2014.12.002
ID 35009
ER

EF