FN Archimer Export Format
PT J
TI Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
BT 
AF BOULAIS, Myrina
   CORPOREAU, Charlotte
   HUVET, Arnaud
   BERNARD, Ismael
   QUERE, Claudie
   QUILLIEN, Virgile
   FABIOUX, Caroline
   SUQUET, Marc
AS 1:1;2:2;3:2;4:1;5:2;6:2;7:3;8:1;
FF 1:PDG-RBE-PFOM-PI;2:PDG-RBE-PFOM-PI;3:PDG-RBE-PFOM-PI;4:;5:PDG-RBE-PFOM-PI;6:PDG-RBE-PFOM-PI;7:;8:PDG-RBE-PFOM-PI;
C1 IFREMER, LEMAR, UMR 6539, F-29840 Argenton, France.
   IFREMER, LEMAR, UMR 6539, F-29280 Plouzane, France.
   IUEM UBO, LEMAR, UMR 6539, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UBO, FRANCE
SI BREST
   ARGENTON
SE PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.893
TC 19
UR https://archimer.ifremer.fr/doc/00239/35010/33544.pdf
LA English
DT Article
DE ;Crassostrea gigas;Oocyte quality;Trochophore;Lipid;Gene expression;ATP
AB Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1 to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R2 = 0.237) and with trochophore Velocity of the Average Path (VAP; R2 = 0.255) and negatively correlated with oocyte total steryl esters (R2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R2 = 0.352), highlighting that the prediction of embryo development in molluscs is a complex process requiring sophisticated multi-factor models.
PY 2015
PD FEB
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 437
UT 000348740100025
BP 201
EP 207
DI 10.1016/j.aquaculture.2014.11.025
ID 35010
ER

EF