FN Archimer Export Format PT J TI Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp. strain BT AF LELCHAT, Florian COZIEN, Joelle LE COSTAOUEC, Tinaig BRANDILY, Christophe SCHMITT, Sophie BAUDOUX, Anne-Claire COLLIEC-JOUAULT, Sylvia BOISSET, Claire AS 1:1,2;2:1,2;3:3;4:1,2;5:1,2;6:4;7:1,2;8:1,2,3; FF 1:;2:PDG-RBE-SG2M-LSEM;3:;4:PDG-REM-EEP-LEP;5:PDG-ODE-DYNECO-PELAGOS;6:;7:PDG-RBE-BRM-LEMMMB;8:PDG-RBE-BRM-BMM; C1 IFREMER, F-29280 Plouzane, France. IFREMER, F-44311 Nantes 3, France. CERMAV, F-38049 Grenoble 9, France. CNRS, Stn Biol Roscoff, F-29680 Roscoff, France. C2 IFREMER, FRANCE IFREMER, FRANCE CERMAV, FRANCE CNRS, FRANCE SI BREST NANTES SE PDG-RBE-SG2M-LSEM PDG-REM-EEP-LEP PDG-ODE-DYNECO-PELAGOS PDG-RBE-BRM-LEMMMB PDG-RBE-BRM-BMM IN WOS Ifremer jusqu'en 2018 copubli-france IF 3.376 TC 17 UR https://archimer.ifremer.fr/doc/00240/35129/33893.pdf LA English DT Article CR HYDRONAUT BO Le Nadir DE ;Marine Alteromonas macleodii sp strain;Bacterial exopolysaccharides;Microbial extracellular enzymes;Endogenous enzymatic degradation AB Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they specifically cleave glycosidic bonds and do not require harsh chemical conditions. The HYD657 EPS is structurally complex and no commercially available enzymes are able to effectively degrade it. Here, we present the first results on the endogenous enzymatic depolymerization of a marine EPS of biotechnological interest by the producing strain. Enzymatic activity was detected in the bacterial lysate and was able to decrease the apparent molecular size of the EPS, releasing mainly oligosaccharides. The reduced form of the native polysaccharide showed a slightly modified osidic composition, particularly in terms of molar ratio. Several exoglycosidase activities were measured in the bacterial lysate using paranitrophenyl-osides PY 2015 PD MAR SO Applied Microbiology And Biotechnology SN 0175-7598 PU Springer VL 99 IS 6 UT 000350362500015 BP 2637 EP 2647 DI 10.1007/s00253-014-6075-y ID 35129 ER EF