FN Archimer Export Format PT J TI Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus BT AF GOUDENEGE, David BOURSICOT, Vincent VERSIGNY, Typhaine BONNETOT, Sandrine RATISKOL, Jacqueline SINQUIN, Corinne LAPOINTE, Gisele LE ROUX, Frederique DELBARRE-LADRAT, Christine AS 1:2,3,4;2:1;3:1;4:1;5:1;6:1;7:5;8:2,3,4;9:1; FF 1:PDG-RBE-PFOM;2:PDG-RBE-BRM-BMM;3:PDG-RBE-BRM-BMM;4:PDG-RBE-BRM-LEMMMB;5:PDG-RBE-BRM-LEMMMB;6:PDG-RBE-BRM-LEMMMB;7:;8:PDG-RBE-PFOM;9:PDG-RBE-BRM-LEMMMB; C1 Ifremer, Laboratoire EM3B (Ecosystèmes Marins et Molécules Marines pour les Biotechnologies), Rue de l’Ile d’Yeu, BP21105, F-44311, Nantes Cedex 3, France Ifremer, Unité Physiologie Fonctionnelle des Organismes Marins, ZI de la Pointe du Diable, CS 10070, F-29280, Plouzané, France Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France Université Laval, Institute for Nutrition and Functional Foods, 2440 boul. Hochelaga, Québec, Québec, Canada C2 IFREMER, FRANCE IFREMER, FRANCE UNIV PARIS 06, FRANCE CNRS, FRANCE UNIV LAVAL, CANADA SI ROSCOFF NANTES SE PDG-RBE-PFOM PDG-RBE-BRM-BMM PDG-RBE-BRM-LEMMMB IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe TC 19 UR https://archimer.ifremer.fr/doc/00241/35244/33892.pdf LA English DT Article DE ;Genome;Vibrio;Exopolysaccharide;Biosynthesis AB Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the glycosidic structure, which may confer an adaptability to environmental conditions. These results contribute to better understanding exopolysaccharide biosynthesis and for developing new efficient processes to produce this molecule for biotechnological applications. PY 2014 PD DEC SO Applied Microbiology And Biotechnology SN 1432-0614 VL 98 IS 24 UT 000345331700021 DI 10.1007/s00253-014-6086-8 ID 35244 ER EF