FN Archimer Export Format
PT J
TI Characterization of the Secretomes of Two Vibrios Pathogenic to Mollusks
BT 
AF MADEC, Stephanie
   PICHEREAU, Vianney
   JACQ, Annick
   PAILLARD, Mathieu
   BOISSET, Claire
   GUERARD, Fabienne
   PAILLARD, Christine
   NICOLAS, Jean-Louis
AS 1:1,5;2:2;3:3;4:2,5;5:4,5;6:2;7:2;8:2,5;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:PDG-RBE-PFOM-PI;5:PDG-RBE-BRM-BMM;6:;7:;8:PDG-RBE-PFOM-PI;
C1 UBO, Lab Univ Biodivers & Ecol Microbienne EA3882, ScInBios SFR48, UEB,ESIAB, F-29280 Plouzane, France.
   UBO, UMR UBO CNRS IRD Ifremer 6539, Lab Sci Environm Marin, Inst Univ Europeen Mer, F-29280 Plouzane, France.
   Univ Paris 11, CNRS, UMR8621, Inst Genet & Microbiol, F-91405 Orsay, France.
   CERMAV CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble, France.
   Ifremer, France
C2 UBO, FRANCE
   UBO, FRANCE
   UNIV PARIS 11, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
SI BREST
   ORSAY
SE PDG-RBE-PFOM-PI
   UBO-LEMAR
   PDG-RBE-BRM-BMM
   UMR-LEMAR
   UBOLEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.234
TC 13
UR https://archimer.ifremer.fr/doc/00242/35334/34576.pdf
LA English
DT Article
AB Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes philippinarum while Vibrio aestuarianus is associated with massive oyster mortalities. As extracellular proteins are often associated with the virulence of pathogenic bacteria, we undertook a proteomic approach to characterize the secretomes of both vibrios. The extracellular proteins (ECPs) of both species were fractionated by SEC-FPLC and in vitro assays were performed to measure the effects of each fraction on hemocyte cellular parameters (phagocytosis and adhesion). Fractions showing a significant effect were subjected to SDS-PAGE, and proteins were identified by nano LC-MS/MS. 45 proteins were identified for V. aestuarianus and 87 for V. tapetis. Most of them belonged to outer membrane or were periplasmic, including porins or adhesins that were already described as virulence factors in other bacterial species. Others were transporter components, flagella proteins, or proteins of unknown function (14 and 15 respectively). Interestingly, for V. aestuarianus, we noted the secretion of 3 extracellular enzymes including the Vam metalloprotease and two other enzymes (one putative lipase and one protease). For V. tapetis, we identified five extracellular enymes, i.e. two different endochitinases, one protease, one lipase and an adhesin. A comparison of both secretomes also showed that only the putative extracellular lipase was common to both secretomes, underscoring the difference in pathogenicity mechanisms between these two species. Overall, these results characterize for the first time the secretomes of these two marine pathogenic vibrios and constitute a useful working basis to further analyze the contribution of specific proteins in the virulence mechanisms of these species.
PY 2014
PD NOV
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 11
UT 000345158700111
BP 1
EP 15
DI 10.1371/journal.pone.0113097
ID 35334
ER

EF