FN Archimer Export Format
PT J
TI Populations, not clones, are the unit of vibrio pathogenesis in naturally infected oysters
BT 
AF LEMIRE, Astrid
   GOUDENEGE, David
   VERSIGNY, Tiphaine
   PETTON, Bruno
   CALTEAU, Alexandra
   LABREUCHE, Yannick
   LE ROUX, Frederique
AS 1:1,2,3;2:1,2,3;3:1,2,3;4:2;5:4,5;6:1,2,3;7:1,2,3;
FF 1:;2:PDG-RBE-PFOM;3:PDG-RBE-BRM-BMM;4:PDG-RBE-PFOM-PI;5:;6:PDG-RBE-PFOM;7:PDG-RBE-PFOM;
C1 Univ Paris 06, Univ Paris 04, Integrat Biol Marine Models Stn Biol Roscoff, UMR 8227, Roscoff, France.
   IFREMER, Unite Physiol Fonctionnelle Organismes Marins, ZI Pointe Diable, Plouzane, France.
   CNRS, Integrat Biol Marine Models Stn Biolog Roscoff, UMR 8227, Roscoff, France.
   CEA, IG, DSV, Evry, France.
   CNRS, LABGeM, UMR 8030, Evry, France.
C2 UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
   CEA, FRANCE
   CNRS, FRANCE
SI ROSCOFF
   NANTES
   ARGENTON
SE PDG-RBE-PFOM
   PDG-RBE-BRM-BMM
   PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 9.328
TC 101
UR https://archimer.ifremer.fr/doc/00244/35565/34107.pdf
LA English
DT Article
AB Disease in oysters has been steadily rising over the past decade, threatening the long-term survival of commercial and natural stocks. Our understanding and management of such diseases are of critical importance as aquaculture is an important aspect of dealing with the approaching worldwide food shortage. Although some bacteria of the Vibrio genus isolated from diseased oysters have been demonstrated to be pathogenic by experimental infection, direct causality has not been established. Little is known about the dynamics of how the bacterial population hosted by oysters changes during disease progression. Combining experimental ecology, a high-throughput infection assay and genome sequencing, we show that the onset of disease in oysters is associated with progressive replacement of diverse benign colonizers by members of a phylogenetically coherent virulent population. Although the virulent population is genetically diverse, all members of that population can cause disease. Comparative genomics across virulent and nonvirulent populations identified candidate virulence factors that were clustered in population-specific genomic regions. Genetic analyses revealed that one gene for a candidate virulent factor, a putative outer membrane protein, is necessary for infection of oysters. Finally, analyses of oyster mortality following experimental infection suggest that disease onset can be facilitated by the presence of nonvirulent strains. This is a new form of polymicrobial disease, in which nonpathogenic strains contribute to increase mortality. 
PY 2015
PD JUN
SO Isme Journal
SN 1751-7362
PU Nature Publishing Group
VL 9
IS 7
UT 000356778000005
BP 1523
EP 1531
DI 10.1038/ismej.2014.233
ID 35565
ER

EF