FN Archimer Export Format PT J TI Beta-N-Methylamino-L-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon BT AF REVEILLON, Damien ABADIE, Eric SECHET, Veronique BRIENT, Luc SAVAR, Veronique BARDOUIL, Michele HESS, Philipp AMZIL, Zouher AS 1:1;2:2;3:1;4:3;5:1;6:1;7:1;8:1; FF 1:PDG-ODE-LITTORAL-PHYC;2:PDG-ODE-LITTORAL-LERLR;3:PDG-ODE-LITTORAL-PHYC;4:;5:PDG-ODE-LITTORAL-PHYC;6:PDG-ODE-LITTORAL-PHYC;7:PDG-ODE-LITTORAL-PHYC;8:PDG-ODE-LITTORAL-PHYC; C1 IFREMER, Phycotoxins Lab, F-44311 Nantes, France. IFREMER, LER LR, F-34203 Sete, France. Univ Rennes 1, UMR Ecobio, F-35042 Rennes, France. C2 IFREMER, FRANCE IFREMER, FRANCE UNIV RENNES, FRANCE SI NANTES SETE SE PDG-ODE-LITTORAL-PHYC PDG-ODE-LITTORAL-LERLR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.853 TC 42 UR https://archimer.ifremer.fr/doc/00245/35579/34112.pdf LA English DT Article DE ;cyanotoxins;BMAA;DAB;AEG;HILIC-MS/MS;cyanobacteria;bivalve mollusks;French Mediterranean AB beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral transitions allowed for the development of a highly selective and sensitive analytical procedure to identify and quantify BMAA and its isomers (in both free and total form) in cyanobacteria and mollusk matrices (LOQ of 0.225 and 0.15 mu g/g dry weight, respectively). Ten species of cyanobacteria (six are reported to be BMAA producers) were screened with this method, and neither free nor bound BMAA could be found, while both free and bound DAB were present in almost all samples. Mussels and oysters collected in 2009 in the Thau Lagoon, France, were also screened, and bound BMAA and its two isomers, DAB and AEG, were observed in all samples (from 0.6 to 14.4 mu g/g DW), while only several samples contained quantifiable free BMAA. PY 2014 PD NOV SO Marine Drugs SN 1660-3397 PU Mdpi Ag VL 12 IS 11 UT 000345531400007 BP 5441 EP 5467 DI 10.3390/md12115441 ID 35579 ER EF