FN Archimer Export Format PT J TI High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring:the eastern English Channel BT AF BONATO, Simon CHRISTAKI, Urania LEFEBVRE, Alain LIZON, Fabrice THYSSEN, Melilotus ARTIGAS, Luis Felipe AS 1:1;2:1;3:2;4:3;5:4;6:1; FF 1:;2:;3:PDG-ODE-LITTORAL-LERBL;4:;5:;6:; C1 Univ Littoral Cote dOpale, Lab Oceanol & Geosci, CNRS UMR LOG 8187, MREN ULCO, F-62930 Wimereux, France. Ifremer LER Boulogne, Lab Environm Littoral & Ressoumes Aquacoles, F-62321 Boulogne Sur Mer, France. Univ Sci & Technol Lille Lille 1, Lab Oceanol & Geosci, CNRS UMR LOG 8187, Stn Marine Wimereux, F-62930 Wimereux, France. Aix Marseille Univ, MIO, UM110, CNRS INSU IRD, F-13288 Marseille 09, France. C2 UNIV LITTORAL COTE D'OPALE, FRANCE IFREMER, FRANCE UNIV LILLE, FRANCE UNIV AIX MARSEILLE, FRANCE SI BOULOGNE SE PDG-ODE-LITTORAL-LERBL IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.335 TC 23 UR https://archimer.ifremer.fr/doc/00248/35885/34406.pdf https://archimer.ifremer.fr/doc/00248/35885/35253.pdf LA English DT Article CR DYPHYMA I ET II BO Côtes De La Manche Sepia II DE ;phytoplankton dynamics;high spatial resolution;flow cytometer;red fluorescence AB The distribution of phytoplankton (from pico-to microphytoplankton) was investigated, at single-cell level and at high spatial resolution, during an oceanographic cruise across the eastern English Channel (EEC) between April 27 and 29, 2012. Seawater was continuously collected from surface waters and analysed on board at high frequency (one sample every 10 min), by using a new generation of pulse-shape recording scanning flow cytometer (CytoSense, Cytobuoy©). A Bray-Curtis matrix analysis based on phytoplankton composition allowed the discrimination of 4 communities. Within these communities, abundance, cell size as well as single cell and total red fluorescence of 8 phytoplankton groups were measured. Picoeukaryotes and Synechococcus spp. cells dominated the mid Channel and most of the English waters monitored, whereas waters off Eastbourne as well as French coastal waters (under remote and direct estuarine influence) were characterized by the dominance of Phaeocystis globosa haploid and diploid cells. Most of the total red fluorescence signal, which correlated with chlorophyll a concentrations, was attributable to P. globosa and, to a lesser extent, to diatoms. In addition to sub-mesoscale variation within phytoplankton communities, the single-cell features within each phytoplankton group gave information about the physiological status of individual phytoplankton cells. PY 2015 PD MAR SO Estuarine Coastal And Shelf Science SN 0272-7714 PU Academic Press Ltd- Elsevier Science Ltd VL 154 UT 000351973100025 BP 214 EP 223 DI 10.1016/j.ecss.2014.12.037 ID 35885 ER EF