FN Archimer Export Format
PT J
TI Identification and separation of saxitoxins using hydrophilic interaction liquid chromatography coupled to traveling wave ion mobility-mass spectrometry
BT 
AF POYER, Salome
   LOUTELIER-BOURHIS, Corinne
   COADOU, Gael
   MONDEGUER, Florence
   ENCHE, Julien
   BOSSEE, Anne
   HESS, Philipp
   AFONSO, Carlos
AS 1:1,2;2:1,2;3:1,2;4:3;5:4;6:4;7:3;8:1,2;
FF 1:;2:;3:;4:PDG-ODE-LITTORAL-PHYC;5:;6:;7:PDG-ODE-LITTORAL-PHYC;8:;
C1 Normandie Univ, COBRA, UMR 6014, F-76821 Mont St Aignan, France.
   Univ Rouen, INSA Rouen, CNRS, IRCOF,FR 3038, F-76821 Mont St Aignan, France.
   IFREMER, Lab Phycotoxines, F-44311 Nantes 03, France.
   DGA Maitrise NRBC, Dept Anal Chim, F-91710 Vert Le Petit, France.
C2 UNIV CAEN NORMANDIE, FRANCE
   UNIV ROUEN, FRANCE
   IFREMER, FRANCE
   DGA MAITRISE NRBC, FRANCE
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.541
TC 22
UR https://archimer.ifremer.fr/doc/00249/36067/34924.pdf
LA English
DT Article
DE ;saxitoxin;ion mobility-mass spectrometry;isomers;multidimensional separation;HILIC
AB The aim of this work was to develop a reliable and efficient analytical method to characterise and differentiate saxitoxin analogues (STX), including sulphated (gonyautoxins, GTX) and non-sulphated analogues. For this purpose, hydrophilic interaction liquid chromatography (HILIC) was used to separate sulphated analogues. We also resorted to ion mobility spectrometry to differentiate the STX analogues because this technique adds a new dimension of separation based on ion gas phase conformation. Positive and negative ionisation modes were used for gonyautoxins while positive ionisation mode was used for non-sulphated analogues. Subsequently, the coupling of these three complementary techniques, HILIC-IM-MS, permitted the separation and identification of STX analogues; isomer differentiation was achieved in HILIC dimension while non-sulphated analogues were separated in the IM-MS dimension. Additional structural characteristics concerning the conformation of STXs could be obtained using IM-MS measurements. Thus, the collision cross sections (CCS) of STXs are reported for the first time in the positive ionisation mode. These experimental CCSs correlated well with the calculated CCS values using the trajectory method.
PY 2015
PD JAN
SO Journal Of Mass Spectrometry
SN 1076-5174
PU Wiley-blackwell
VL 50
IS 1
UT 000348721800024
BP 175
EP 181
DI 10.1002/jms.3515
ID 36067
ER

EF