Inhibition mechanism of Listeria monocytogenes by a bioprotective bacteria Lactococcus piscium CNCM I-4031

Type Article
Date 2016-02
Language English
Author(s) Saraoui Taous1, 2, 4, Fall Papa-Abdoulaye1, 2, 4, Leroi Francoise1, Antignac Jean-Philippe3, Chereau Sylvain3, Pilet Marie France2, 4
Affiliation(s) 1 : IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol E, F-44311 Nantes 03, France.
2 : LUNAM Univ, Oniris, Secalim UMR 1014, F-44307 Nantes, France.
3 : LUNAM Univ, Oniris, USC INRA 1329, Lab Etud Residus & Contaminants Aliments LABERCA, F-44307 Nantes, France.
4 : INRA, F-44307 Nantes, France.
Source Food Microbiology (0740-0020) (Academic Press Ltd- Elsevier Science Ltd), 2016-02 , Vol. 53 , P. 70-78
DOI 10.1016/j.fm.2015.01.002
WOS© Times Cited 29
Note 19th Meeting of the Club des Bactéries Lactiques
Keyword(s) Listeria monocytogenes, Lactococcus piscium, Nutrients competition, Cellular contact, Chemically defined medium, Bacterial interaction
Abstract Listeria monocytogenes is a pathogenic Gram positive bacterium and the etiologic agent of listeriosis, a severe food-borne disease. Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp. To investigate the inhibititory mechanism, a chemically defined medium (MSMA) based on shrimp composition and reproducing the inhibition observed in shrimp was developed. In co-culture at 26°C, L. monocytogenes was reduced by 3-4 log CFU g-1 after 24 h. We have demonstrated that the inhibition was not due to secretion of extracellular antimicrobial compounds as bacteriocins, organic acids and hydrogen peroxide. Global metabolomic fingerprints of these strains in pure culture were assessed by liquid chromatography coupled with high resolution mass spectrometry. Consumption of glucose, amino-acids, vitamins, nitrogen bases, iron and magnesium was measured and competition for some molecules could be hypothesized. However, after 24 h of co-culture, when inhibition of L. monocytogenes occurred, supplementation of the medium with these compounds did not restore its growth. The inhibition was observed in co-culture but not in diffusion chamber when species were separated by a filter membrane. Taken together, these data indicate that the inhibition mechanism of L. monocytogenes by L. piscium is cell-to-cell contact-dependent.
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