FN Archimer Export Format
PT J
TI Successful detection of pathogenic Shiga-toxin-producing Escherichia coli in shellfish, environmental waters and sediment using the ISO/TS-13136 method
BT 
AF BALIERE, Charlotte
   RINCE, Alain
   THEVENOT, Delphine
   GOURMELON, Michele
AS 1:1;2:2;3:3;4:1;
FF 1:PDG-RBE-SG2M-LSEM;2:;3:;4:PDG-RBE-SG2M-LSEM;
C1 Unité SG2M, Département RBE, Laboratoire Santé Environnement et Microbiologie, IFREMER, Plouzané, France
   UCBN, U2RM Stress/Virulence EA4655, Normandie-Université, Caen, France
   Laboratoire LMAP-LNR, UMR5557/Equipe 6, Marcy-L'Etoile, France
C2 IFREMER, FRANCE
   UNIV CAEN NORMANDIE, FRANCE
   LABORATOIRE LMAP-LNR, FRANCE
SI BREST
SE PDG-RBE-SG2M-LSEM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.579
TC 14
UR https://archimer.ifremer.fr/doc/00250/36124/34975.pdf
LA English
DT Article
DE ;eae;sediment;shellfish;Shiga-toxin-producing Escherichia coli;stx;water
AB The presence of highly pathogenic Shiga-toxin-producing Escherichia coli (STEC) in shellfish, upstream waters and sediment from coastal shellfish sites was evaluated using the ISO/TS-13136 method. Shellfish (oysters, mussels and cockles), water and sediment samples were collected monthly over a period of 1 year. The method used real-time PCR detection of stx1, stx2 and eae genes and genetic markers corresponding to the five major serogroups (O157, O26, O103, O111 and O145) on enrichment broths and the identification of STEC when these genes and markers were detected. stx genes were detected in the broth of 33% of shellfish batches (n = 126), 91% of water samples (n = 117) and 28% of sediment (n = 39). One stx1+, eae+ O26:H11 strain was isolated from a shellfish batch, and O26:H11, O145:H28 and O103:H2 strains without the stx gene (n = 9) were isolated from shellfish and waters. In conclusion, this study shows the suitability of the ISO/TS-13136 method to assess the presence of highly pathogenic E. coli strains in shellfish farming areas. It also highlights a low prevalence of STEC and consequently suggests a reduced corresponding human health risk.
PY 2015
PD APR
SO Letters in Applied Microbiology
SN 0266-8254
PU Wiley / Blackwell
VL 60
IS 4
UT 000351395000002
DI 10.1111/lam.12386
ID 36124
ER

EF