TY - JOUR T1 - Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae A1 - Mersni-Achour,Rachida A1 - Ben Cheikh,Yosra A1 - Pichereau,Vianney A1 - Doghri,Ibtissem A1 - Etien,Cedric A1 - Degremont,Lionel A1 - Saulnier,Denis A1 - Fruitier-Arnaudin,Ingrid A1 - Travers,Marie-Agnes AD - Univ La Rochelle, CNRS ULR, LIENSs, Equipe Approches Mol,Environm Sante,UMR 7266, F-17042 La Rochelle, France. AD - IFREMER, SG2M LGPMM, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France. AD - Univ La Rochelle, Federat Rech Environm & Dev Durable, FR CNRS 3097, La Rochelle, France. AD - Univ Europeenne Bretagne, Lab Sci Environnement Mann LEMAR, CNRS,IFREMER,UBO,IRD, Inst Univ Europeen Mer,Univ Bretagne Occidentale, F-29280 Plouzane, France. AD - IFREMER, Ctr Iftemer Pacifique, UMR 241, Ecosysternes Lnsulaires Oceaniens, Tahiti 98719, Taravao, Fr Polynesia. UR - https://doi.org/10.1099/mic.0.000058 DO - 10.1099/mic.0.000058 N2 - Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs causing bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with a LD50 about 2.3 × 103 cfu ml-1 after 24 h. Moreover, complete or GP- HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECPs fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease Y1 - 2015/05 PB - Soc General Microbiology JF - Microbiology-sgm SN - 1350-0872 VL - 161 SP - 997 EP - 1007 ID - 36367 ER -