FN Archimer Export Format PT J TI Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae BT AF MERSNI-ACHOUR, Rachida BEN CHEIKH, Yosra PICHEREAU, Vianney DOGHRI, Ibtissem ETIEN, Cedric DEGREMONT, Lionel SAULNIER, Denis FRUITIER-ARNAUDIN, Ingrid TRAVERS, Marie-Agnes AS 1:1,2,3;2:1;3:4;4:1;5:1;6:2;7:2,5;8:1,3;9:2; FF 1:;2:;3:;4:;5:;6:PDG-RBE-SG2M-LGPMM;7:PDG-RBE-RMPF;8:;9:PDG-RBE-SG2M-LGPMM; C1 Univ La Rochelle, CNRS ULR, LIENSs, Equipe Approches Mol,Environm Sante,UMR 7266, F-17042 La Rochelle, France. IFREMER, SG2M LGPMM, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France. Univ La Rochelle, Federat Rech Environm & Dev Durable, FR CNRS 3097, La Rochelle, France. Univ Europeenne Bretagne, Lab Sci Environnement Mann LEMAR, CNRS,IFREMER,UBO,IRD, Inst Univ Europeen Mer,Univ Bretagne Occidentale, F-29280 Plouzane, France. IFREMER, Ctr Iftemer Pacifique, UMR 241, Ecosysternes Lnsulaires Oceaniens, Tahiti 98719, Taravao, Fr Polynesia. C2 UNIV LA ROCHELLE, FRANCE IFREMER, FRANCE UNIV LA ROCHELLE, FRANCE UBO, FRANCE IFREMER, FRANCE SI BREST LA TREMBLADE TAHITI SE UBOLEMAR PDG-RBE-SG2M-LGPMM PDG-RBE-RMPF UM LEMAR EIO IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.268 TC 21 UR https://archimer.ifremer.fr/doc/00252/36367/34907.pdf LA English DT Article AB Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs causing bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with a LD50 about 2.3 × 103 cfu ml-1 after 24 h. Moreover, complete or GP- HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECPs fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease PY 2015 PD MAY SO Microbiology-sgm SN 1350-0872 PU Soc General Microbiology VL 161 UT 000358186900008 BP 997 EP 1007 DI 10.1099/mic.0.000058 ID 36367 ER EF