FN Archimer Export Format
PT J
TI Microwave-assisted extraction of phycobiliproteins from Porphyridium purpureum
BT 
AF JUIN, Camille
   CHEROUVRIER, Jean-Rene
   THIERY, Valerie
   GAGEZ, Anne-Laure
   BERARD, Jean-Baptiste
   JOGUET, Nicolas
   KAAS, Raymond
   CADORET, Jean-Paul
   PICOT, Laurent
AS 1:1;2:1;3:1;4:1;5:2;6:1;7:2;8:2;9:1;
FF 1:;2:;3:;4:;5:PDG-RBE-BRM-PBA;6:;7:PDG-RBE-BRM-PBA;8:PDG-RBE-BRM;9:;
C1 UMRi CNRS 7266 LIENSs University of La Rochelle, 17042, La Rochelle, France
   IFREMER Laboratoire BRM/PBA, Rue de l’Ile d’Yeu, 44311, Nantes, France
C2 UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
   PDG-RBE-BRM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.606
TC 52
UR https://archimer.ifremer.fr/doc/00253/36439/35169.pdf
LA English
DT Article
DE ;Allophycocyanin;MAE;Microalgae;Microwave;Phycobiliprotein;Phycocyanin;Phycoerythrin;Porphyridium;Thylakoid
AB In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 A degrees C, and PE was thermally damaged at temperatures higher than 40 A degrees C. In contrast, a flash irradiation for 10 s at 100 A degrees C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary to extract them from the thylakoid membrane to a short exposure to thermal denaturation. The extraction order of the three phycobiliproteins was coherent with the structure of Pp phycobilisomes. Moreover, the absorption and fluorescence properties of MAE extracted phycobiliproteins were stable for several months after the microwave treatment. Scanning electron microscopy indicated that MAE at 100 A degrees C induced major changes in the Pp cell morphology, including fusion of the exopolysaccharidic cell walls and cytoplasmic membranes of adjacent cells. As a conclusion, MAE is a fast and high yield process efficient to extract and pre-purify phycobiliproteins, even from microalgae containing a thick exopolysaccharidic cell wall.
PY 2015
PD JAN
SO Applied Biochemistry and Biotechnology
SN 0273-2289
PU Humana Press
VL 175
IS 1
UT 000348102900001
BP 1
EP 15
DI 10.1007/s12010-014-1250-2
ID 36439
ER

EF