FN Archimer Export Format PT J TI A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors BT AF NASFI, H. TRAVERS, Marie-Agnes DE LORGERIL, Julien HABIB, C. SANNIE, T. SORIEUL, L. GERARD, J. AVARRE, J. C. HAFFNER, Philippe TOURBIEZ, Delphine RENAULT, Tristan FURONES, D. ROQUE, A. PRUZZO, C. CHESLETT, D. GDOURA, R. VALLAEYS, T. AS 1:1;2:2;3:3;4:4;5:1;6:1;7:1;8:5;9:2;10:2;11:2;12:6;13:6;14:7;15:8;16:9;17:1; FF 1:;2:PDG-RBE-SG2M-LGPMM;3:PDG-RBE-IHPE;4:;5:;6:;7:;8:;9:PDG-RBE-SG2M-LGPMM;10:PDG-RBE-SG2M-LGPMM;11:PDG-RBE;12:;13:;14:;15:;16:;17:; C1 CC 093 UMR 5119 “Ecologie des Systèmes Marins Côtiers”, Université de Montpellier 2, place Eugène Bataillon, 34095, Montpellier, France Laboratoire de Génétique et Pathologie des Mollusque Marins, Institut Français de Recherche pour l’Exploitation de la Mer, av. du Mus de Loup, 17390, La Tremblade, France UMR 5119 “Ecologie des Systèmes Marins Côtiers”, Institut Français de Recherche pour l’Exploitation de la Mer, pl E. Bataillon, 34095, Montpellier, France UR1077, Mathématique Informatique et Génome, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78350, Jouy-en-Josas, France UMR 226, ISEM, Institut Recherche pour le Développement, pl E. Bataillon, 34095, Montpellier, France IRTA-Sant Carles de la Ràpita, Crta. Poble Nou, km. 5, 5, 43540, Tarragona, Spain 7. Department of Life, Earth and Environmental Sciences (DISTAV), University of Genoa, Genoa, Italy Department of Life, Earth and Environmental Sciences (DISTAV), University of Genoa, Genoa, Italy Fish Health Unit, Marine Institute, Rinville, Oranmore, Co., Galway, Ireland UR11ES70, Unité de recherche Toxicologie Microbiologie Environnementale et Santé, Faculté des sciences de Sfax, Université de Sfax, B.P. 1171, 3000, Sfax, Tunisia C2 UNIV MONTPELLIER, FRANCE IFREMER, FRANCE IFREMER, FRANCE INRA, FRANCE IRD, FRANCE IRTA, ITALY UNIV GENOA, ITALY MARINE INST GALWAY, IRELAND UNIV SFAX, TUNISIA SI LA TREMBLADE MONTPELLIER NANTES SE PDG-RBE-SG2M-LGPMM PDG-RBE-IHPE PDG-RBE UM IHPE IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 copubli-europe copubli-univ-france copubli-int-hors-europe copubli-sud IF 1.532 TC 20 UR https://archimer.ifremer.fr/doc/00255/36666/35350.pdf LA English DT Article DE ;VNTR;Vibrio splendidus;Epidemiology;vsm;ompU;Crassotrea giga;Mortality AB The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase chain reaction with 16S specific primers and probes. A multiple-locus variable-number tandem-repeat analysis (VNTR) was conducted on all isolates based on a multiplex PCR-VNTR with a set of primer pairs designed from the V. tasmaniensis LGP32 genome. Preliminary validation of the primers on a set of collection strains from the V. splendidus clade confirmed that the former V. splendidus-related LGP32 and relative strains were related to V. tasmaniensis rather than to the type strain V. splendidus LMG 4042. The VNTR analysis was then successfully conducted on 335 isolates which led to the characterization of 87 different profiles. Our results showed that (1) the high diversity of VNTR did not enlighten significant correlation between a specific pattern and the origin of collected samples. However, populations isolated from animal samples tend to differ from those of the background environment; (2) oyster mortality events could not be linked to the clonal proliferation of a particular VNTR type. However, few different patterns seemed successively associated with samples collected during peaks of oyster's mortality. (3) Finally, no correlation could be seen between specific VNTR patterns and sequence phylogeny of the virulence factors vsm and ompU that were detected among strains isolated during as well as outside mortality events. These results, combined with incongruence observed between the ompU and vsm phylogenetic trees, suggested both large diffusion of strains and massive lateral gene transfer within the V. splendidus clade. PY 2015 PD MAR SO World Journal of Microbiology and Biotechnology SN 0959-3993 PU Springer VL 31 IS 3 UT 000349978200004 BP 461 EP 475 DI 10.1007/s11274-015-1800-y ID 36666 ER EF