FN Archimer Export Format PT J TI Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps BT AF VIGNERON, Adrien L HARIDON, Stephane GODFROY, Anne ROUSSEL, Erwan CRAGG, Barry A. PARKES, R. John TOFFIN, Laurent AS 1:1,2,3,5;2:2,3;3:1,2,3;4:1,2,3;5:4;6:4;7:1,2,3; FF 1:PDG-REM-EEP-LMEE;2:;3:PDG-REM-EEP-LMEE;4:PDG-REM-EEP-LMEE;5:;6:;7:PDG-REM-EEP-LMEE; C1 IFREMER, Lab Microbiol Environm Extremes, Technopole Brest Iroise, UMR6197, Plouzane, France. Univ Bretagne Occidentale, Lab Microbiol Environm Extremes, Technopole Brest Iroise, UMR6197, Plouzane, France. CNRS, Lab Microbiol Environm Extremes, Technopole Brest Iroise, UMR6197, Plouzane, France. Cardiff Univ, Sch Earth & Ocean Sci, Cardiff CF10 3AX, S Glam, Wales. Newcastle Univ, Sch Civil Engn & Geosci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. C2 IFREMER, FRANCE UBO, FRANCE CNRS, FRANCE UNIV CARDIFF, UK UNIV NEWCASTLE, UK SI BREST SE PDG-REM-EEP-LMEE UM BEEP-LM2E IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 3.823 TC 25 UR https://archimer.ifremer.fr/doc/00256/36737/35338.pdf LA English DT Article CR BIG BO L'Atalante AB In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various microbial communities, such as abundant lineages of anaerobic methanotrophs (ANME). However, the biodiversity, distribution, and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabeled dimethylamine, bicarbonate, and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of denaturing gradient gel electrophoresis (DGGE) fingerprinting and reverse-transcribed PCR-amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages, confirming the cooccurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps. PY 2015 PD MAY SO Applied And Environmental Microbiology SN 0099-2240 PU Amer Soc Microbiology VL 81 IS 10 UT 000353336900018 BP 3451 EP 3459 DI 10.1128/AEM.00147-15 ID 36737 ER EF