FN Archimer Export Format
PT J
TI Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera
BT 
AF TEANINIURAITEMOANA, Vaihiti
   HUVET, Arnaud
   LEVY, Peva
   GAERTNER-MAZOUNI, Nabila
   GUEGUEN, Yannick
   LE MOULLAC, Gilles
AS 1:1;2:2;3:1;4:3;5:1,4;6:1;
FF 1:PDG-RBE-RMPF;2:PDG-RBE-PFOM-PI;3:PDG-RBE-RMPF;4:;5:PDG-RBE-IHPE;6:PDG-RBE-RMPF;
C1 IFREMER, UMR Ecosyst Insulaires Oceaniens EIO 241, Labex Corail, Ctr Pacifique, Taravao 98719, Tahiti, Fr Polynesia.
   IFREMER, UMR Lab Sci Environm Marin LEMAR 6539, F-29280 Plouzane, France.
   Univ Polynesie Francaise, UMR Ecosyst Insulaires Oceaniens EIO 241, Labex Corail, Faaa 98702, Tahiti, Fr Polynesia.
   Univ Montpellier, IFREMER, UMR Ecol Syst Marins Cotiers ECOSYM 5119, Labex Corail, F-34095 Montpellier 5, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV POLYNESIE FRANCAISE, FRANCE
   UNIV MONTPELLIER, FRANCE
SI TAHITI
   BREST
   MONTPELLIER
SE PDG-RBE-RMPF
   PDG-RBE-PFOM-PI
   PDG-RBE-IHPE
UM LEMAR
   EIO
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.057
TC 20
UR https://archimer.ifremer.fr/doc/00256/36768/35374.pdf
LA English
DT Article
AB The genomics of economically important marine bivalves is studied to provide better understanding of the molecular mechanisms underlying their different reproductive strategies. The recently available gonad transcriptome of the black-lip pearl oyster Pinctada margaritifera is a novel and powerful resource to study these mechanisms in marine mollusks displaying hermaphroditic features. In this study, RNAseq quantification data of the P. margaritifera gonad transcriptome were analyzed to identify candidate genes in histologically-characterized gonad samples to provide molecular signatures of the female and male sexual pathway in this pearl oyster. Based on the RNAseq data set, stringent expression analysis identified 1,937 contigs that were differentially expressed between the gonad histological categories. From the hierarchical clustering analysis, a new reproduction model is proposed, based on a dual histo-molecular analytical approach. Nine candidate genes were identified as markers of the sexual pathway: 7 for the female pathway and 2 for the male one. Their mRNA levels were assayed by real-time PCR on a new set of gonadic samples. A clustering method revealed four principal expression patterns based on the relative gene expression ratio. A multivariate regression tree realized on these new samples and validated on the previously analyzed RNAseq samples showed that the sexual pathway of P. margaritifera can be predicted by a 3-gene-pair expression ratio model of 4 different genes: pmarg-43476, pmarg-foxl2, pmarg-54338 and pmarg-fem1-like. This 3-gene-pair expression ratio model strongly suggests only the implication of pmarg-foxl2 and pmarg-fem1-like in the sex inversion of P. margaritifera. This work provides the first histo-molecular model of P. margaritifera reproduction and a gene expression signature of its sexual pathway discriminating the male and female pathways. These represent useful tools for understanding and studying sex inversion, sex differentiation and sex determinism in this species and other related species for aquaculture purposes such as genetic selection programs.
PY 2015
PD MAR
SO Plos One
SN 1932-6203
PU Public Library Science
VL 10
IS 3
UT 000352133600200
DI 10.1371/journal.pone.0122819
ID 36768
ER

EF