FN Archimer Export Format
PT J
TI The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae
BT 
AF RUMIN, Judith
   BONNEFOND, Hubert
   SAINT-JEAN, Bruno
   ROUXEL, Catherine
   SCIANDRA, Antoine
   BERNARD, Olivier
   CADORET, Jean-Paul
   BOUGARAN, Gael
AS 1:1;2:2,4;3:1;4:1;5:2,4;6:3;7:1;8:1;
FF 1:PDG-RBE-BRM-PBA;2:;3:PDG-RBE-BRM-PBA;4:PDG-RBE-BRM-PBA;5:;6:;7:PDG-RBE-BRM;8:PDG-RBE-BRM-PBA;
C1 IFREMER, PBA, F-44311 Nantes, France.
   CNRS, UMPC, LOV UMR 7093, F-06230 Villefranche Sur Mer, France.
   INRIA BIOCORE, F-06902 Sophia Antipolis, France.
C2 IFREMER, FRANCE
   CNRS, FRANCE
   INRIA, FRANCE
   UNIV PARIS 06, FRANCE
SI NANTES
   AUTRE
SE PDG-RBE-BRM-PBA
   AUTRE
   PDG-RBE-BRM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 6.444
TC 257
UR https://archimer.ifremer.fr/doc/00257/36823/35451.pdf
LA English
DT Article
DE ;Nile red;BODIPY 505/515;Microalgae;Neutral lipid;Fluorescence;Biodiesel
AB Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification.
PY 2015
PD MAR
SO Biotechnology For Biofuels
SN 1754-6834
PU Biomed Central Ltd
VL 8
IS 42
UT 000351122400001
BP 1
EP 16
DI 10.1186/s13068-015-0220-4
ID 36823
ER

EF