Parental trophic exposure to three aromatic fractions of polycyclic aromatic hydrocarbons in the zebrafish: Consequences for the offspring

Type Article
Date 2015-08
Language English
Author(s) Perrichon Prescilla1, 2, Akcha Farida1, Le Menach Karyn3, Goubeau Manon1, Budzinski Helene3, Cousin XavierORCID1, 4, Bustamante Paco2
Affiliation(s) 1 : IFREMER, Lab Ecotoxicol, Lhoumeau Nantes, France.
2 : Univ La Rochelle, Littoral Environm & Soc LIENSs, CNRS, UMRi 7266, F-17042 La Rochelle 01, France.
3 : Univ Bordeaux, EPOC, LPTC, UMR CNRS 5805, F-33405 Talence, France.
4 : INRA, Lab Physiol & Genom Poisons, F-35042 Rennes, France.
Source Science Of The Total Environment (0048-9697) (Elsevier Science Bv), 2015-08 , Vol. 524 , P. 52-62
DOI 10.1016/j.scitotenv.2015.04.018
WOS© Times Cited 18
Keyword(s) Polycyclic aromatic hydrocarbons, Parental transmission, Fish early-life stages, PhotoMotor Response, Cardiac performance, PAH metabolites, Genotoxicity
Abstract In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6 months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3 ×, 1 × and 3 ×; 1 × represents an environmental concentration measured in French estuary). In order to explore the consequences of parental exposure for the next first generation, toxic responses were studied in both embryos and larvae using a multiscale approach. Toxic effects were assessed by looking at hatching success, developmental abnormalities, photomotor response and heartbeat. The level of PAH metabolites and EROD activity in fish larvae were measured to assess exposure to PAHs. Egg production of parents was significantly reduced compared to the Control; hence little information was available for BAL and HFO offspring. The size of larvae from PY parents was found to increase despite a reduced yolk sac compared to Control larvae. Furthermore, a high level of behavioral stress was observed in larvae originating from parents exposed to three-fold the environmental concentration. The cardiac activity was reduced in a concentration-dependent manner for the PY exposure group. No effect was however observed on biotransformation markers (cyp1a, EROD), nor on the level of DNA damage for all PY, BAL and HFO offspring. The absence of significant differences in metabolite levels may indicate a potential early depuration of transferred compounds or no PAH-transmission. The disruptions observed at the individual level in the next generation could impact on the longer-term, surviving population
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Perrichon Prescilla, Akcha Farida, Le Menach Karyn, Goubeau Manon, Budzinski Helene, Cousin Xavier, Bustamante Paco (2015). Parental trophic exposure to three aromatic fractions of polycyclic aromatic hydrocarbons in the zebrafish: Consequences for the offspring. Science Of The Total Environment, 524, 52-62. Publisher's official version : , Open Access version :