FN Archimer Export Format
PT J
TI A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus
BT 
AF DE SOUSA, Joana Teixeira
   MILAN, Massimo
   PAULETTO, Marianna
   BARGELLONI, Luca
   JOAQUIM, Sandra
   MATIAS, Domitilia
   MATIAS, Ana Margarete
   QUILLIEN, Virgile
   LEITAO, Alexandra
   HUVET, Arnaud
AS 1:1,1,2,2;2:3,3;3:3,3;4:3,3;5:2,2,4,4;6:2,2,4,4;7:2,2;8:1,1;9:2,2,5,5;10:1,1;
FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-RBE-PFOM-PI;9:;10:PDG-RBE-PFOM-PI;
C1 IFREMER, Lab Sci Environm Marin, UMR CNRS 6539, F-29280 Plouzane, France.
   IPMA, P-8700305 Olhao, Portugal.
   Univ Padua, Dept Comparat Biomed & Food Sci, I-35020 Legnaro, Italy.
   Univ Porto, CIIMAR, Interdisciplinary Ctr Marine & Environm Res, P-4050123 Oporto, Portugal.
   Qatar Univ, Ctr Environm Studies, Doha, Qatar.
C2 IFREMER, FRANCE
   IPMA, PORTUGAL
   UNIV PADUA, ITALY
   UNIV PORTO, PORTUGAL
   UNIV QATAR, QATAR
SI BREST
SE PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-europe
   copubli-int-hors-europe
IF 1.893
TC 6
UR https://archimer.ifremer.fr/doc/00260/37147/35676.pdf
LA English
DT Article
DE ;Ruditapes decussatus;Oocyte;Developmental success;Gene expression;Marine bivalve;Transcriptomics
AB A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment analysis showed “Lysosome” (dre04142) as the single pathway represented in the enriched KEGG pathway terms, with 8 genes coding for putative Cathepsins. Furthermore, differentially expressed genes involved in oocyte protection (DnaJ (Hsp40), Hsp70, Cyclophilin B, PDI), maturation (Cam-PDE1C, PRDM9, G protein), sperm-egg interaction (PDI, G protein) and apoptosis (TNF) were also identified. From these, the apoptosis pathway was supposed to assume an important role in oocyte quality as the mRNA level of Caspase 8 appeared also negatively correlated with the D-larval yield. Finally, a G-protein transcript was identified as more abundant in poor quality oocytes, which underlines the importance of release prophase I block and meiotic maturation in the oocytes of this species. This study provided new highly valuable information on genes specifically expressed by mature oocytes of R. decussatus, for further understanding of the mechanisms of early development in this species.
PY 2015
PD SEP
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 446
UT 000355672000004
BP 17
EP 24
DI 10.1016/j.aquaculture.2015.04.018
ID 37147
ER

EF