FN Archimer Export Format
PT J
TI Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
BT 
AF DA COSTA GONZALEZ, Fiz
   ROBERT, Rene
   QUERE, Claudie
   WIKFORS, Gary H.
   SOUDANT, Philippe
AS 1:1,2;2:1,3;3:1;4:4;5:5;
FF 1:;2:PDG-ODE-LITTORAL;3:PDG-RBE-PFOM-PI;4:;5:;
C1 Ifremer/Laboratoire des sciences de l’Environnement Marin (UMR 6539, LEMAR), 29280, Plouzané, France
   Novostrea Bretagne, Route du Vieux Passage, Banastère, 56370, Sarzeau, France
   IFREMER, Unite Littoral, Ctr Bretagne, F-29280 Plouzane, France.
   NOAA, Northeast Fisheries Sci Ctr, NMFS, Milford, CT 06460 USA.
   IUEM UBO, LEMAR, Lab Sci Environm Marin UMR 6539, Plouzane, France.
C2 IFREMER, FRANCE
   NOVOSTREA BRETAGNE, FRANCE
   IFREMER, FRANCE
   NOAA, USA
   UBO, FRANCE
SI BREST
SE PDG-ODE-LITTORAL
   PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 1.892
TC 37
UR https://archimer.ifremer.fr/doc/00268/37962/36086.pdf
LA English
DT Article
DE ;Fatty acid;Larvae;Lipids;Metabolism;Oyster;Synthesis
AB Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5 desaturase. Moreover, this Delta 5 desaturase presumably allowed larvae to convert 20:3n-6 and 20:4n-3 to 20:4n-6 and 20:5n-3, respectively, when the product EFA were poorly or not supplied in the diet, as when larvae were fed T exclusively. Under our experimental conditions, none of the diets induced 22:6n-3 synthesis; however, 22:6n-3 incorporation into larval tissues occurred selectively under non-limiting dietary supply to maintain optimal levels in the larvae. This combination of flow-through larval rearing and biochemical analysis of FA levels could be applied to additional dietary experiments to precisely define optimal levels of EFA supply.
PY 2015
PD MAY
SO Lipids
SN 0024-4201
PU Springer Heidelberg
VL 50
IS 5
UT 000353566200007
BP 503
EP 511
DI 10.1007/s11745-015-4006-z
ID 37962
ER

EF