TY - JOUR T1 - UPLC-MSE Profiling of Phytoplankton Metabolites: Application to the Identification of Pigments and Structural Analysis of Metabolites in Porphyridium purpureum A1 - Juin,Camille A1 - Bonnet,Antoine A1 - Nicolau,Elodie A1 - Berard,Jean-Baptiste A1 - Devillers,Romain A1 - Thiery,Valerie A1 - Cadoret,Jean-Paul A1 - Picot,Laurent AD - Univ La Rochelle, UMRi CNRS LIENSs 7266, F-17042 La Rochelle, France. AD - Univ La Rochelle, UMRi CNRS LIENSs 7266, Platform High Resolut Anal Biomol, F-17042 La Rochelle, France. AD - IFREMER, Lab BRM PBA, F-44311 Nantes, France. UR - https://archimer.ifremer.fr/doc/00269/37994/ DO - 10.3390/md13042541 KW - carotenoid KW - chlorophyll KW - dereplication KW - divinyl chlorophyll a KW - galactosyldiacylglycerol KW - gracilamide KW - mass spectrometry KW - MSE KW - phytoplankton KW - pigment KW - Porphyridium purpureum KW - UPLC N2 - A fast and high-resolution UPLC-MSE analysis was used to identify phytoplankton pigments in an ethanol extract of Porphyridium purpureum (Pp) devoid of phycobiliproteins. In a first step, 22 standard pigments were analyzed by UPLC-MSE to build a database including retention time and accurate masses of parent and fragment ions. Using this database, seven pigments or derivatives previously reported in Pp were unequivocally identified: beta,beta-carotene, chlorophyll a, zeaxanthin, chlorophyllide a, pheophorbide a, pheophytin a, and cryptoxanthin. Minor amounts of Divinyl chlorophyll a, a chemotaxonomic pigment marker for prochlorophytes, were also unequivocally identified using the database. Additional analysis of ionization and fragmentation patterns indicated the presence of ions that could correspond to hydroxylated derivatives of chlorophyll a and pheophytin a, produced during the ethanolic extraction, as well as previously described galactosyldiacylglycerols, the thylakoid coenzyme plastoquinone, and gracilamide B, a molecule previously reported in the red seaweed Gracillaria asiatica. These data point to UPLC-MSE as an efficient technique to identify phytoplankton pigments for which standards are available, and demonstrate its major interest as a complementary method for the structural elucidation of ionizable marine molecules. Y1 - 2015 PB - Mdpi Ag JF - Marine Drugs SN - 1660-3397 VL - 13 IS - 4 SP - 2541 EP - 2558 ID - 37994 ER -