FN Archimer Export Format PT J TI UPLC-MSE Profiling of Phytoplankton Metabolites: Application to the Identification of Pigments and Structural Analysis of Metabolites in Porphyridium purpureum BT AF JUIN, Camille BONNET, Antoine NICOLAU, Elodie BERARD, Jean-Baptiste DEVILLERS, Romain THIERY, Valerie CADORET, Jean-Paul PICOT, Laurent AS 1:1;2:2;3:3;4:3;5:1;6:1;7:3;8:1; FF 1:;2:;3:PDG-RBE-BRM-PBA;4:PDG-RBE-BRM-PBA;5:;6:;7:PDG-RBE-BRM;8:; C1 Univ La Rochelle, UMRi CNRS LIENSs 7266, F-17042 La Rochelle, France. Univ La Rochelle, UMRi CNRS LIENSs 7266, Platform High Resolut Anal Biomol, F-17042 La Rochelle, France. IFREMER, Lab BRM PBA, F-44311 Nantes, France. C2 UNIV LA ROCHELLE, FRANCE UNIV LA ROCHELLE, FRANCE IFREMER, FRANCE SI NANTES SE PDG-RBE-BRM-PBA PDG-RBE-BRM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.345 TC 22 UR https://archimer.ifremer.fr/doc/00269/37994/36069.pdf https://archimer.ifremer.fr/doc/00269/37994/36070.pdf LA English DT Article DE ;carotenoid;chlorophyll;dereplication;divinyl chlorophyll a;galactosyldiacylglycerol;gracilamide;mass spectrometry;MSE;phytoplankton;pigment;Porphyridium purpureum;UPLC AB A fast and high-resolution UPLC-MSE analysis was used to identify phytoplankton pigments in an ethanol extract of Porphyridium purpureum (Pp) devoid of phycobiliproteins. In a first step, 22 standard pigments were analyzed by UPLC-MSE to build a database including retention time and accurate masses of parent and fragment ions. Using this database, seven pigments or derivatives previously reported in Pp were unequivocally identified: beta,beta-carotene, chlorophyll a, zeaxanthin, chlorophyllide a, pheophorbide a, pheophytin a, and cryptoxanthin. Minor amounts of Divinyl chlorophyll a, a chemotaxonomic pigment marker for prochlorophytes, were also unequivocally identified using the database. Additional analysis of ionization and fragmentation patterns indicated the presence of ions that could correspond to hydroxylated derivatives of chlorophyll a and pheophytin a, produced during the ethanolic extraction, as well as previously described galactosyldiacylglycerols, the thylakoid coenzyme plastoquinone, and gracilamide B, a molecule previously reported in the red seaweed Gracillaria asiatica. These data point to UPLC-MSE as an efficient technique to identify phytoplankton pigments for which standards are available, and demonstrate its major interest as a complementary method for the structural elucidation of ionizable marine molecules. PY 2015 SO Marine Drugs SN 1660-3397 PU Mdpi Ag VL 13 IS 4 UT 000353715900049 BP 2541 EP 2558 DI 10.3390/md13042541 ID 37994 ER EF