FN Archimer Export Format
PT J
TI Structure Elucidation, Relative LC–MS Response and In Vitro Toxicity of Azaspiracids 7–10 Isolated from Mussels (Mytilus edulis)
BT 
AF KILCOYNE, Jane
   TWINER, Michael J.
   MCCARRON, Pearse
   CRAIN, Sheila
   GIDDINGS, Sabrina D.
   FOLEY, Barry
   RISE, Frode
   HESS, Philipp
   WILLDNS, Alistair L.
   MILES, Christopher O.
AS 1:1,2;2:3;3:4;4:4;5:4;6:2;7:5;8:6;9:7;10:7,8;
FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-ODE-LITTORAL-PHYC;9:;10:;
C1 Inst Marine, Oranmore, County Galway, Ireland.
   Dublin Inst Technol, Sch Chem & Pharmaceut Sci, Dublin 8, Ireland.
   Wayne State Univ, Sch Med, Detroit, MI 48202 USA.
   Natl Res Council Canada, Measurement Sci & Stand Biotoxin Metrol, Halifax, NS B3H 3Z1, Canada.
   Univ Oslo, Dept Chem, N-0315 Oslo, Norway.
   Ifremer, Lab Phycotoxines, F-44311 Nantes, France.
   Norwegian Vet Inst, N-0106 Oslo, Norway.
   Univ Oslo, Sch Pharm, Dept Pharmaceut Chem, N-0316 Oslo, Norway.
C2 MARINE INST GALWAY, IRELAND
   DUBLIN INST TECHNOL, IRELAND
   UNIV WAYNE STATE, USA
   NRC, CANADA
   UNIV OSLO, NORWAY
   IFREMER, FRANCE
   NORWEGIAN VET INST, NORWAY
   UNIV OSLO, NORWAY
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-europe
   copubli-int-hors-europe
IF 2.857
TC 31
UR https://archimer.ifremer.fr/doc/00269/38059/36190.pdf
LA English
DT Article
DE ;azaspiracid;structure confirmation;LC-MS molar response;NMR;mass spectrometry;purification;Jurkat T;toxicity
AB Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7–10, 7–10, were isolated from shellfish and their structures, previously proposed on the basis of only LC–MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4–6, 4–6, and 7–10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC–MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a time- and concentration-dependent manner; however, there were distinct differences in their EC50 values, with the potencies for each analogue being: AZA6 > AZA8 > AZA1 > AZA4 ≈ AZA9 > AZA5 ≈ AZA10. This data contributes to the understanding of the structure–activity relationships of AZAs.
PY 2015
PD MAY
SO Journal Of Agricultural And Food Chemistry
SN 0021-8561
PU Amer Chemical Soc
VL 63
IS 20
UT 000355383700020
BP 5083
EP 5091
DI 10.1021/acs.jafc.5b01320
ID 38059
ER

EF