FN Archimer Export Format
PT J
TI Rearing effect of biofloc on antioxidant and antimicrobial transcriptional response in Litopenaeus stylirostris shrimp facing an experimental sub-lethal hydrogen peroxide stress
BT 
AF CARDONA, Emilie
   SAULNIER, Denis
   LORGEOUX, Benedicte
   CHIM, Liet
   GUEGUEN, Yannick
AS 1:1,2;2:1;3:1;4:2;5:1,3;
FF 1:;2:PDG-RBE-RMPF;3:;4:PDG-RBE-LEADNC;5:PDG-RBE-IHPE;
C1 IFREMER, Unite Rech Resources Marines, Ctr Oceanolog Pacifique, Taravao 98719, Fr Polynesia.
   IFREMER, Unite Rech Lagons Ecosyst & Aquaculture Durable N, Noumea 98846, New Caledonia.
   Univ Montpellier, CNRS, UPVD, Ifremer,UMR IHPE 5244, F-34095 Montpellier, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
SI TAHITI
   NOUMEA
   MONTPELLIER
SE PDG-RBE-RMPF
   PDG-RBE-LEADNC
   PDG-RBE-IHPE
UM IHPE
   EIO
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.025
TC 39
UR https://archimer.ifremer.fr/doc/00270/38116/36269.pdf
LA English
DT Article
DE ;Biofloc;Shrimps;Antimicrobial peptides;Antioxidant enzymes
AB This study compares the antioxidant and antimicrobial transcriptional expression of blue shrimps reared according to two different systems, BioFloc Technology (BFT) and Clear sea Water (CW) and their differential responses when facing an experimental sublethal hydrogen peroxide stress. After 30 days of rearing, juvenile shrimps were exposed to H2O2 stress at a concentration of 30 ppm during 6 hours. The oxidative stress caused by H2O2 was examined in the digestive glands of the shrimp, in which antioxidant enzyme (AOE) and antimicrobial peptide (AMP) gene expression were analysed by quantitative real-time PCR. Results showed that rearing conditions did not affect the expression of genes encoding AOEs or AMPs. However, H2O2 stress induced a differential response in expression between shrimps from the two rearing treatments (BFT and CW). Comparative analysis of the expression profiles indicates that catalase transcripts were significantly upregulated by H2O2 stress for BFT shrimps while no change was observed for CW shrimps. In contrast, H2O2 caused down-regulation of superoxide dismutase and glutathione transferase transcripts and of the three AMP transcripts studied (penaeidin 2 and 3, and crustin) for CW shrimps, while no effect was observed on BFT shrimp transcript levels. These results suggested that BFT shrimps maintained antioxidant and AMP responses after stress and therefore can effectively protect their cells against oxidative stress, while CW shrimp immune competence seems to decrease after stress.
PY 2015
PD AUG
SO Fish & Shellfish Immunology
SN 1050-4648
PU Academic Press Ltd- Elsevier Science Ltd
VL 45
IS 2
UT 000358626800084
BP 933
EP 939
DI 10.1016/j.fsi.2015.05.041
ID 38116
ER

EF