FN Archimer Export Format PT J TI Induction of apoptosis by UV in the flat oyster, Ostrea edulis BT AF GERVAIS, Ophelie RENAULT, Tristan ARZUL, Isabelle AS 1:1;2:1;3:1; FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE;3:PDG-RBE-SG2M-LGPMM; C1 IFREMER, LGPMM, Unite Sante Genet & Microbiol Mollusques SG2M, F-17390 La Tremblade, France. C2 IFREMER, FRANCE SI LA TREMBLADE NANTES SE PDG-RBE-SG2M-LGPMM PDG-RBE IN WOS Ifremer jusqu'en 2018 IF 3.025 TC 21 UR https://archimer.ifremer.fr/doc/00270/38160/36295.pdf LA English DT Article DE ;Apoptosis;Hemocyte(s);Oyster;Ostrea edulis;UV;Flow cytometry;Microscopy AB Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that apoptosis occurs in flat oyster hemocytes. Investigated parameters like intracytoplasmic calcium activity, mitochondrial membrane potential and phosphatidyl-serine externalization were significantly modulated in cells exposed to UV whereas dexamethasone only induced an increase of DNA fragmentation. Morphological changes were also observed on UV-treated cells using fluorescence microscopy and transmission electron microscopy. Our results confirm the apoptotic effect of UV on hemocytes of O. edulis and suggest that apoptosis is an important mechanism developed by the flat oyster against stress factors. PY 2015 PD OCT SO Fish & Shellfish Immunology SN 1050-4648 PU Academic Press Ltd- Elsevier Science Ltd VL 46 IS 2 UT 000363071200009 BP 232 EP 242 DI 10.1016/j.fsi.2015.05.046 ID 38160 ER EF