FN Archimer Export Format
PT J
TI A Method for Detection of Trace Concentrations of Underivatized Amino Acid in Hydrothermal Fluids by Ion-Pairing Reversed-Phase UPLC-ESI-QTOF-MS
BT 
AF KONN, Cecile
   MAGNER, Jörgen
   CHARLOU, Jean-Luc
   HOLM, Nils G
   ALSBERG, Tomas
AS 1:1,3;2:2,4;3:3;4:1;5:2;
FF 1:PDG-REM-GM-LGM;2:;3:PDG-REM-GM-LGM;4:;5:;
C1 Department of Geological Sciences, Stockholm University, SE-10691 Stockholm, Sweden
   Department of Environmental Science and Analytical Chemistry (ACES), Stockholm University, SE-10691 Stockholm, Sweden
   Ifremer, Laboratoire de Géochimieet Métallogénie, F-29280 Plouzané, France
   IVL Swedish Environmental Research Institute Ltd., SE-10031 Stockholm, Sweden
C2 UNIV STOCKHOLM, SWEDEN
   UNIV STOCKHOLM, SWEDEN
   IFREMER, FRANCE
   IVL, SWEDEN
SI BREST
SE PDG-REM-GM-LGM
TC 0
UR https://archimer.ifremer.fr/doc/00271/38188/36339.pdf
LA English
DT Article
CR MOMAR2008-LEG1
   MOMAR2008-LEG2
   MOMARDREAM-NAUT1-NAUT2
   SERPENTINE
BO L'Atalante
   Pourquoi pas ?
DE ;Amino Acids;UPLC;Ion-Pairing;QTOF;Mass Spectrometry;Hydrothermal Fluids
AB Investigation of amino acids in hydrothermal systems is of prime importance for the understanding of geochemistry and microbiology of hydrothermal vents and plumes, for carbon and metals global cycles, for metabolism of some hydrothermal microorganisms and for the origin of life issue. Extensive theoretical and experimental work on amino acids behaviour in hydrothermal fluids has been done, conversely only few data exist on natural samples. Because each hydrothermal vent is unique, the more data we collect the better we will be able to address each of these questions. Usually amino acids in hydrothermal fluids have been measured by HPLC-FLD. The chromatographic separation was at least 26 min and up to 135 min and the required derivatization step may be time consuming, may use harmful chemicals and may be source of contamination. Alternatively, we describe here a method combining quickness (4.5 min), high resolution (10,000), very low LOD (sub-ppb) and without derivatization. Characterisation and separation of 10 relevant proteinogenic underivatized amino acids was achieved by ion-pairing reversed-phase Ultra-high Performance Liquid Chromatography-Electrospray Ionisation-Quadrupole Time of Flight-Mass Spectrometry (UPLC-ESI-QTOF-MS). Excellent linearity in the response was obtained for all amino acids with correlation coefficients > 0.9921. This method was successfully applied to natural hydrothermal fluid samples from ultramafic-hosted vents of the Mid-Atlantic Ridge region. Results are consistent with the only 2 other studies published on ultramafic-hosted vents and complete the few available data.
PY 2015
PD MAR
SO American Journal of Analytical Chemistry
SN 2156-8251
PU Sicentific Research Publishing
VL 6
BP 313
EP 324
DI 10.4236/ajac.2015.64030
ID 38188
ER

EF