FN Archimer Export Format
PT J
TI Disruption of amylase genes by RNA interference affects reproduction in the Pacific oyster Crassostrea gigas
BT 
AF HUVET, Arnaud
   BEGUEL, Jean-Philippe
   PEREIRA CAVALEIRO, Nathalia
   THOMAS, Yoann
   QUILLIEN, Virgile
   BOUDRY, Pierre
   ALUNNO-BRUSCIA, Marianne
   FABIOUX, Caroline
AS 1:1;2:2;3:3;4:4;5:1;6:1;7:1;8:2;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:;5:PDG-RBE-PFOM-PI;6:PDG-RBE-PFOM;7:PDG-RBE-PFOM-PI;8:;
C1 IFREMER, UMR CNRS UBO IRD Ifremer 6539, Lab Sci Environm Marin, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, Inst Univ Europeen de la Mer, UMR CNRS UBO IRD Ifremer 6539, Lab Sci Environm Marin, F-29280 Plouzane, France.
   Lab Nacl Comp Cient, BR-25651071 Rio De Janeiro, Brazil.
   Univ Nantes, Mer Mol Sante EA 2160, F-44322 Nantes 3, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   LNCC, BRAZIL
   UNIV NANTES, FRANCE
SI BREST
   ARGENTON
SE PDG-RBE-PFOM-PI
   PDG-RBE-PFOM
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.914
TC 34
UR https://archimer.ifremer.fr/doc/00271/38239/36422.pdf
LA English
DT Article
DE ;alpha-Amylase;dsRNA;Gametogenesis;Marine bivalve;Dynamic energy budget
AB Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15 and 75 µg dsRNA-injected oysters, respectively, relative to controls. Interestingly, reproductive knock-down phenotypes were observed for both sexes at 48 days post-injection, with a significant reduction of the gonad area (−22.5% relative to controls) and germ cell under-proliferation revealed by histology. In response to the higher dose of dsRNA, we also observed reductions in amylase activity (−53%) and absorption efficiency (−5%). Based on these data, dynamic energy budget modeling showed that the limitation of energy intake by feeding that was induced by injection of amylase dsRNA was insufficient to affect gonadic development at the level observed in the present study. This finding suggests that other driving mechanisms, such as endogenous hormonal modulation, might significantly change energy allocation to reproduction, and increase the maintenance rate in oysters in response to dsRNA injection.
PY 2015
PD JUL
SO Journal Of Experimental Biology
SN 0022-0949
PU Company Of Biologists Ltd
VL 218
IS 11
UT 000355602000023
BP 1740
EP 1747
DI 10.1242/jeb.116699
ID 38239
ER

EF