Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc: Assessment by C and N stable isotope ratios and effect on key digestive enzymes

Type Article
Date 2015-11
Language English
Author(s) Cardona Emilie1, 2, Lorgeoux Benedicte1, Geffroy Claire2, Richard Pierre3, Saulnier Denis1, Gueguen YannickORCID1, 4, Guillou Gael3, Chim Liet2
Affiliation(s) 1 : IFREMER, Ctr Oceanol Pacifique, Unite Rech Ressources Marines, Taravao 98719, Fr Polynesia.
2 : IFREMER, Ecosyst & Aquaculture Durable Nouvelle Caledonie, Unite Rech Lagons, Noumea 98846, New Caledonia.
3 : Univ La Rochelle, Lab Littoral Environm & Soc, CNRS, UMR 6250, F-17000 La Rochelle, France.
4 : Univ Montpellier, CNRS, IFREMER, UMR IHPE 5244,UPVD, F-34095 Montpellier, France.
Source Aquaculture (0044-8486) (Elsevier Science Bv), 2015-11 , Vol. 448 , P. 288-297
DOI 10.1016/j.aquaculture.2015.05.035
WOS© Times Cited 30
Keyword(s) Biofloc, Shrimps, Stable isotope, Digestive enzyme activities, Digestive enzyme mRNA level
Abstract The aim of this study was to assess the relative contribution of natural productivity and compound food to the growth of the juvenile blue shrimp Litopenaeus stylirostris reared in a biofloc system. Two experiments were carried out based on the same protocol with three treatments: clear water with experimental diet (CW), biofloc with experimental diet (BF) and biofloc unfed (BU). Shrimp survival was significantly higher in biofloc rearing than in CW rearing. The contribution of the biofloc to shrimp diet was estimated through measurement of carbon and nitrogen stable isotope ratios in shrimp and food sources. Different isotopic compositions between feeds were obtained by feeding natural productivity with a mixture rich in fish meal and the shrimps with a pellet containing a high level of soy protein concentrate. Using a two source one-isotope mixing model, we found that the natural productivity of the biofloc system contributed to shrimp growth at a level of 39.8% and 36.9%, for C and N, respectively. The natural food consumed by the shrimps reared in the biofloc system resulted in higher gene expression (mRNA transcript abundance) and activities of two digestive enzymes in their digestive gland: α-amylase and trypsin. The growth of shrimp biomass reared in biofloc was, on average, 4.4 times that of those grown in clear water. Our results confirmed the best survival and promoted growth of shrimps using biofloc technology and highlighted the key role of the biofloc in the nutrition of rearing shrimps.
Statement of relevance

In this study, we have applied an original protocol to determine the respective contribution of natural productivity and artificial feeds on the alimentation of the juvenile blue shrimp L. stylirostris reared in biofloc system by using C and N natural stable isotope analysis. Moreover, we have compared, in shrimp digestive gland, the α-amylase and trypsin enzyme activities at biochemical and molecular levels for two different shrimp rearing systems, biofloc and clear water. In our knowledge, the use of molecular tool to study the influence of biofloc consumption on digest process of shrimp was never carried out. We think that our research is new and important to increase knowledge on biofloc topic.
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Cardona Emilie, Lorgeoux Benedicte, Geffroy Claire, Richard Pierre, Saulnier Denis, Gueguen Yannick, Guillou Gael, Chim Liet (2015). Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc: Assessment by C and N stable isotope ratios and effect on key digestive enzymes. Aquaculture, 448, 288-297. Publisher's official version : https://doi.org/10.1016/j.aquaculture.2015.05.035 , Open Access version : https://archimer.ifremer.fr/doc/00271/38242/