FN Archimer Export Format PT J TI Use of Natural Antimicrobial Peptides and Bacterial Biopolymers for Cultured Pearl Production BT AF SIMON-COLIN, Christelle GUEGUEN, Yannick BACHERE, Evelyne KOUZAYHA, Achraf SAULNIER, Denis GAYET, Nicolas GUEZENNEC, Jean AS 1:1;2:2,3;3:2;4:4;5:3;6:1;7:5; FF 1:PDG-REM-EEP-LMEE;2:PDG-RBE-IHPE;3:PDG-RBE-IHPE;4:PDG-RBE-BOME;5:PDG-RBE-RMPF;6:PDG-REM-EEP-LEP;7:; C1 IFREMER, Ctr Brest, F-29280 Plouzane, France. Univ Montpellier 2, CNRS, UPVD, Ifremer UMR IHPE 5244, F-34095 Montpellier, France. UPF ILM IRD, IFREMER, UMR EIO 241, Labex Corail, Taravao 98719, Fr Polynesia. Lebanese Univ, Sect 3, Dept Biochem, Fac Sci, Tripoli, Lebanon. AiMB, F-29280 Plouzane, France. C2 IFREMER, FRANCE UNIV PERPIGNAN, FRANCE UNIV POLYNESIE FRANCAISE, FRANCE UNIV LEBANESE, LEBANON AIMB, FRANCE SI BREST MONTPELLIER TAHITI SE PDG-REM-EEP-LMEE PDG-RBE-IHPE PDG-RBE-BOME PDG-RBE-RMPF PDG-REM-EEP-LEP UM BEEP-LM2E IHPE EIO IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 3.345 TC 13 UR https://archimer.ifremer.fr/doc/00275/38599/37128.pdf LA English DT Article DE ;pearl oyster;exopolysaccharide;tachyplesin;antimicrobial AB Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with two marine bacterial exopolymers as alternative treatment agents. In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls. The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination. The survival data were similar to that reported for antibiotic treatments. These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures. PY 2015 PD JUL SO Marine Drugs SN 1660-3397 PU Mdpi Ag VL 13 IS 6 UT 000357279900027 BP 3732 EP 3744 DI 10.3390/md13063732 ID 38599 ER EF