FN Archimer Export Format
PT J
TI Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas
BT 
AF FABIOUX, Caroline
   SULISTIYANI, Yeni
   HABERKORN, Hansy
   HEGARET, Helene
   AMZIL, Zouher
   SOUDANT, Philippe
AS 1:1;2:1;3:1;4:1;5:2;6:1;
FF 1:;2:;3:;4:;5:PDG-ODE-LITTORAL-PHYC;6:;
C1 Univ Bretagne Occidentale, Lab Sci Environm Marin LEMAR, Inst Univ European Mer, UMR 6539,CNRS UBO IRD IFREMER, F-29280 Plouzane, France.
   IFREMER, Lab Phycotoxines, F-44311 Nantes, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.664
TC 44
UR https://archimer.ifremer.fr/doc/00275/38601/38623.pdf
LA English
DT Article
DE ;Crassostrea gigas;Alexandrium minutum;Paralytic shellfish toxins;Antioxidant enzymes;Detoxification enzymes;mRNA
AB Harmful algal blooms of Alexandrium spp. dinoflagellates regularly occur in French coastal waters contaminating shellfish. Studies have demonstrated that toxic Alexandrium spp. disrupt behavioural and physiological processes in marine filter-feeders, but molecular modifications triggered by phycotoxins are less well understood. This study analyzed the mRNA levels of 7 genes encoding antioxidant/detoxifying enzymes in gills of Pacific oysters (Crassostrea gigas) exposed to a cultured, toxic strain of A. minutum, a producer of paralytic shellfish toxins (PST) or fed Tisochrysis lutea (T. lutea, formerly Isochrysis sp., clone Tahitian (T. iso)), a non-toxic control diet, in four repeated experiments. Transcript levels of sigma-class glutathione S-transferase (GST), glutathione reductase (GR) and ferritin (Fer) were significantly higher in oysters exposed to A. minutum compared to oysters fed T. lutea. The detoxification pathway based upon glutathione (GSH)-conjugation of toxic compounds (phase II) is likely activated, and catalyzed by GST. This system appeared to be activated in gills probably for the detoxification of PST and/or extra-cellular compounds, produced by A. minutum. GST, GR and Fer can also contribute to antioxidant functions to prevent cellular damage from increased reactive oxygen species (ROS) originating either from A. minutum cells directly, from oyster hemocytes during immune response, or from other gill cells as by-products of detoxification.
PY 2015
PD SEP
SO Harmful Algae
SN 1568-9883
PU Elsevier Science Bv
VL 48
UT 000361251800007
BP 55
EP 62
DI 10.1016/j.hal.2015.07.003
ID 38601
ER

EF