FN Archimer Export Format
PT J
TI Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa
BT 
AF PRADO-ALVAREZ, Maria
   COURALEAU, Yann
   CHOLLET, Bruno
   TOURBIEZ, Delphine
   ARZUL, Isabelle
AS 1:1;2:1;3:1;4:1;5:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-SG2M-LGPMM;5:PDG-RBE-SG2M-LGPMM;
C1 IFREMER, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-RBE-SG2M-LGPMM
IN WOS Ifremer jusqu'en 2018
IF 3.031
TC 4
UR https://archimer.ifremer.fr/doc/00277/38871/37410.pdf
LA English
DT Article
DE ;Bonamia exitiosa;Bonamia ostreae;actin;Haplosporidia;Whole-Genome Amplification
AB Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where the herein described B. exitiosa actin resolved, offering novel information about the classification of the genus. Our results showed that WGA methodology is a promising and valuable technique to be applied to unculturable protozoans whose genomic material is limited.
PY 2015
PD OCT
SO Parasitology
SN 0031-1820
PU Cambridge Univ Press
VL 142
IS 12
UT 000362911100007
BP 1523
EP 1534
DI 10.1017/S0031182015000967
ID 38871
ER

EF